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Literature summary for 3.1.6.12 extracted from

  • Pungor, E.; Hague, C.M.; Chen, G.; Lemontt, J.F.; Dvorak-Ewell, M.; Prince, W.S.
    Development of a functional bioassay for arylsulfatase B using the natural substrates of the enzyme (2009), Anal. Biochem., 395, 144-150.
    View publication on PubMed
Search for more literature for 3.1.6.12

Cloned(Commentary)

Cloned (Comment) Organism
expression of ASB C53S mutant in CHO cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
C53S the mutation causes ASB-deficiency, phenoytpe, overview. The enzyme is taken up into cultured ASB-deficient human fibroblasts, GM00519 cells, and translocates to the lysosomes, it is catalytically active. The enzyme enters target cells predominantly through the CI-M6P receptor. The uptake of rhASB is able to restore lysosomal function in an in vitro cell-based assay Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Homo sapiens 5764
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chondroitin sulfate + H2O Homo sapiens
-
 ?
-
 ?
dermatan sulfate + H2O Homo sapiens
-
 ?
-
 ?
additional information Homo sapiens ASB is a lysosomal exohydrolase, cleaving the 4-sulfate from the N-acetylgalactosamine-4-sulfate residue at the nonreducing terminal of glycosaminoglycan structures ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
fibroblast
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chondroitin sulfate + H2O
-
 Homo sapiens ?
-
 ?
dermatan sulfate + H2O
-
 Homo sapiens ?
-
 ?
additional information ASB is a lysosomal exohydrolase, cleaving the 4-sulfate from the N-acetylgalactosamine-4-sulfate residue at the nonreducing terminal of glycosaminoglycan structures Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
arylsulfatase B
-
 Homo sapiens
ASB
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
 assay at Homo sapiens

General Information

General Information Comment Organism
malfunction ASB-deficient cells accumulate dermatan sulfate and chondroitin sulfate, which may be partially hydrolyzed by other lysosomal hydrolases Homo sapiens