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Literature summary for 3.1.6.12 extracted from

  • Christianson, T.M.; Starr, C.M.; Zankel, T.C.
    Overexpression of inactive arylsulfatase mutants and in vitro activation by light-dependent oxidation with vanadate (2004), Biochem. J., 382, 581-587.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.6.12

Activating Compound

Activating Compound Comment Organism Structure
vanadate maximal activation achieved for mutant arylsulfatase B C91S at highest vanadate concentration of 0.4 mM with concomitant irradation for 120 min Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in CHO-K1 Homo sapiens

Protein Variants

Protein Variants Comment Organism
C91S catalytically inactive enzyme can be converted into an active enzyme form by vanadate and light Homo sapiens
C91S present at significantly higher levels in conditioned media when compared with the wild type Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P15848
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information conversion of an active-site cysteine into a formylglycine Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
mutant C91S purified on a anti-ASB affinity column prepared by coupling the antibody with NHS-sepharose beads Homo sapiens
mutant enzyme C91S Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl sulfate + H2O
-
 Homo sapiens 4-methylumbelliferone + sulfate
-
 ?
4-methylumbelliferyl sulfate + H2O
-
 Homo sapiens 4-methylumbelliferol + sulfate
-
 ?

Synonyms

Synonyms Comment Organism
arylsulfatase B
-
 Homo sapiens