Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.6.12 extracted from

  • Tsuji, M.; Nakanishi, Y.; Habuchi, H.; Ishihara, K.; Suzuki, S.
    The common identity of UDP-N-acetylgalactosamine 4-sulfatase, nitrocatechol sulfatase (arylsulfatase), and chondroitin 4-sulfatase (1980), Biochim. Biophys. Acta, 612, 373-383.
    View publication on PubMed
Search for more literature for 3.1.6.12

General Stability

General Stability Organism
thawing and freezing, stable at -20°C, 6 months with occasional thawing and refreezing Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
Na2HPO4
-
 Gallus gallus
SO42-
-
 Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.43
-
 UDP-N-acetylgalactosamine 4-sulfate
-
 Gallus gallus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 gel filtration Gallus gallus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-D-galactosamine 4-sulfate units + H2O Gallus gallus acts also on N-acetylglucosamine 4-sulfate N-acetyl-D-galactosamine units + sulfate
-
 ?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
oviduct
-
 Gallus gallus
-

Storage Stability

Storage Stability Organism
-20°C, 6 months, with occasional thawing and refreezing, no loss of activity Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chondroitin 4-sulfate + H2O
-
 Gallus gallus chondroitin + sulfate
-
 ?
dermatan sulfate + H2O
-
 Gallus gallus dermatan + sulfate
-
 ?
N-acetyl-D-galactosamine 4-sulfate + H2O
-
 Gallus gallus N-acetyl-D-galactosamine + sulfate
-
 ?
N-acetyl-D-galactosamine 4-sulfate units + H2O acts also on N-acetylglucosamine 4-sulfate Gallus gallus N-acetyl-D-galactosamine units + sulfate
-
 ?
N-acetylgalactosamine 4,6-bisulfate-(beta-glucuronosyl-(1-3)-N-acetylgalactosamine 4-sulfate)3 + H2O hydrolysis only at the nonreducing residues Gallus gallus N-acetylgalactosamine 6-sulfate-(beta-glucuronosyl-(1-3)-N-acetylgalactosamine)3 + sulfate
-
 ?
p-nitrocatechol sulfate + H2O enzymes catalyze in addition to reaction of EC 3.1.6.12 also arylsulfatase reaction , see also EC 3.1.6.1 Gallus gallus p-nitrocatechol + sulfate
-
 ?
p-nitrophenyl sulfate + H2O
-
 Gallus gallus p-nitrophenol + sulfate
-
 ?
UDP-N-acetylgalactosamine 4,6-bisulfate + H2O
-
 Gallus gallus UDP-N-acetylgalactosamine 6-sulfate + sulfate
-
 ?
UDP-N-acetylgalactosamine-4-sulfate + H2O
-
 Gallus gallus UDP-N-acetylgalactosamine + sulfate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Gallus gallus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 20 min Gallus gallus
60
-
 75% loss of activity Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8
-
 chondroitin 4-sulfate Gallus gallus
5
-
 UDP-N-acetylgalactosamine 4-sulfate Gallus gallus
5.6
-
 nitrocatechol sulfate Gallus gallus