Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.6.1 extracted from

  • Zhu, Y.; Liu, H.; Qiao, C.; Li, L.; Jiang, Z.; Xiao, A.; Ni, H.
    Characterization of an arylsulfatase from a mutant library of Pseudoalteromonas carrageenovora arylsulfatase (2017), Int. J. Biol. Macromol., 96, 370-376 .
    View publication on PubMed
Search for more literature for 3.1.6.1

Protein Variants

Protein Variants Comment Organism
H260L mutant displays increased thermostability and increased half-life at 55°C Pseudoalteromonas carrageenovora

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 1 mM, 81% of initial activity, mutant H260L Pseudoalteromonas carrageenovora
Cd2+ 1 mM, 65% of initial activity, mutant H260L Pseudoalteromonas carrageenovora
Cu2+ 1 mM, 9% of initial activity, mutant H260L Pseudoalteromonas carrageenovora
dithiothreitol 1 mM, 69.8% of initial activity, mutant H260L Pseudoalteromonas carrageenovora
EDTA 1 mM, 125.6% of initial activity, mutant H260L Pseudoalteromonas carrageenovora
guanidine hydrochloride 1 mM, 69.5% of initial activity, mutant H260L Pseudoalteromonas carrageenovora
Mg2+ 1 mM, 88% of initial activity, mutant H260L Pseudoalteromonas carrageenovora
Urea 1 mM, 86% of initial activity, mutant H260L Pseudoalteromonas carrageenovora
Zn2+ 1 mM, complete loss of activity, mutant H260L Pseudoalteromonas carrageenovora

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.66
-
 4-nitrophenyl sulfate mutant H260L, pH 7.5, 55°C Pseudoalteromonas carrageenovora
0.71
-
 4-nitrophenyl sulfate wild-type, pH 7.5, 55°C Pseudoalteromonas carrageenovora

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 1 mM, 110% of initial activity, mutant H260L Pseudoalteromonas carrageenovora
Ca2+ 10 mM, 137% of initial activity, mutant H260L Pseudoalteromonas carrageenovora
Co2+ 1 mM, 119% of initial activity, mutant H260L Pseudoalteromonas carrageenovora
Mn2+ 10 mM, 230% of initial activity, mutant H260L Pseudoalteromonas carrageenovora
Na+ 1 mM, 108% of initial activity, mutant H260L Pseudoalteromonas carrageenovora

Organism

Organism UniProt Comment Textmining
Pseudoalteromonas carrageenovora P28607
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl sulfate + H2O
-
 Pseudoalteromonas carrageenovora 4-nitrophenol + sulfate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 mutant H260L Pseudoalteromonas carrageenovora

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 mutant H260L Pseudoalteromonas carrageenovora

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 mutant H260L Pseudoalteromonas carrageenovora