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Literature summary for 3.1.6.1 extracted from

  • Xiao, Q.; Yin, Q.; Ni, H.; Cai, H.; Wu, C.; Xiao, A.
    Characterization and immobilization of arylsulfatase on modified magnetic nanoparticles for desulfation of agar (2017), Int. J. Biol. Macromol., 94, 576-584 .
    View publication on PubMed
Search for more literature for 3.1.6.1

Application

Application Comment Organism
synthesis immobilization of enzyme on carboxyl functioned magnetic nanoparticles using glutaraldehyde. Immobilization leads to increased tolerance to some metal ions, inhibitors and detergents. Immobilized arylsulfatase maintains 61.7% of its initial desulfuration rates after seven cycles. After the reaction of agar with immobilized arylsulfatase for 90 min at 50°C, 46% of the sulfate in the agar is removed Pseudoalteromonas carrageenovora

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 1 mM, 70% residual activity, both free and immobilized enzyme Pseudoalteromonas carrageenovora
Ag+ 1 mM, less than 20% residual activity, both free and immobilized enzyme Pseudoalteromonas carrageenovora
Al3+ 1 mM, less than 20% residual activity, both free and immobilized enzyme Pseudoalteromonas carrageenovora
Cd2+ 1 mM, less than 20% residual activity, both free and immobilized enzyme Pseudoalteromonas carrageenovora
Cu2+ 1 mM, less than 20% residual activity, both free and immobilized enzyme Pseudoalteromonas carrageenovora
Fe2+ 1 mM, less than 20% residual activity, both free and immobilized enzyme Pseudoalteromonas carrageenovora
Fe3+ 1 mM, less than 20% residual activity, both free and immobilized enzyme Pseudoalteromonas carrageenovora
PMSF 1 mM, 15% residual activity, free enzyme, 35% residual activity, immobilized enzyme, respectively Pseudoalteromonas carrageenovora
SDS 1 mM, 5% residual activity, free enzyme, 45% residual activity, immobilized enzyme, respectively Pseudoalteromonas carrageenovora
Triton X-100 1 mM, 15% residual activity, free enzyme, 35% residual activity, immobilized enzyme, respectively Pseudoalteromonas carrageenovora
Tween 80 1 mM, 10% residual activity, free enzyme, 60% residual activity, immobilized enzyme, respectively Pseudoalteromonas carrageenovora
Zn2+ 1 mM, less than 20% residual activity, both free and immobilized enzyme Pseudoalteromonas carrageenovora

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.89
-
 4-nitrophenyl sulfate pH 7.5, 50°C, immobilized enzyme Pseudoalteromonas carrageenovora

Organism

Organism UniProt Comment Textmining
Pseudoalteromonas carrageenovora P28607
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl sulfate + H2O
-
 Pseudoalteromonas carrageenovora 4-nitrophenol + sulfate
-
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Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
256.9
-
 4-nitrophenyl sulfate pH 7.5, 50°C, immobilized enzyme Pseudoalteromonas carrageenovora