Application | Comment | Organism |
---|---|---|
analysis | enzyme hydrolyzes dehydroepiandrosterone 3-sulfate and epiandrosterone 3-sulfate in a urine matrix. Purified arylsulfatase contains only sulfatase activity allowing for the selective hydrolysis of sulfate esters in the presence of glucuronide conjugates. The enzyme can be employed for short three-step chemoenzymatic synthesis of 5alpha-androstane-3beta,17beta-diol 17-glucuronide from epiandrosterone 3-sulfate | Pseudomonas aeruginosa |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
4-nitrophenyl sulfate | pH 8.8, 37°C | Pseudomonas aeruginosa | |
0.014 | - |
Estrone 3-sulfate | pH 8.8, 37°C | Pseudomonas aeruginosa | |
0.026 | - |
epiandrosterone 3-sulfate | pH 8.8, 37°C | Pseudomonas aeruginosa | |
0.05 | - |
testosterone 17-sulfate | pH 8.8, 37°C | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | P51691 | - |
- |
Pseudomonas aeruginosa DSM 22644 | P51691 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl sulfate + H2O | - |
Pseudomonas aeruginosa | 4-nitrophenol + sulfate | - |
? | |
4-nitrophenyl sulfate + H2O | - |
Pseudomonas aeruginosa DSM 22644 | 4-nitrophenol + sulfate | - |
? | |
androsterone 3-sulfate + H2O | reaction of EC 3.1.6.2 | Pseudomonas aeruginosa | androsterone + sulfate | - |
? | |
androsterone 3-sulfate + H2O | reaction of EC 3.1.6.2 | Pseudomonas aeruginosa DSM 22644 | androsterone + sulfate | - |
? | |
boldenone 17-sulfate + H2O | - |
Pseudomonas aeruginosa | boldenone + sulfate | - |
? | |
dehydroepiandrosterone 3-sulfate + H2O | reaction of EC 3.1.6.2 | Pseudomonas aeruginosa | dehydroepiandrosterone + sulfate | - |
? | |
dehydroepiandrosterone 3-sulfate + H2O | reaction of EC 3.1.6.2 | Pseudomonas aeruginosa DSM 22644 | dehydroepiandrosterone + sulfate | - |
? | |
epiandrosterone 3-sulfate + H2O | reaction of EC 3.1.6.2 | Pseudomonas aeruginosa | epiandrosterone + sulfate | - |
? | |
epiandrosterone 3-sulfate + H2O | reaction of EC 3.1.6.2 | Pseudomonas aeruginosa DSM 22644 | epiandrosterone + sulfate | - |
? | |
epitestosterone 17-sulfate + H2O | reaction of EC 3.1.6.2 | Pseudomonas aeruginosa | epitestosterone + sulfate | - |
? | |
estrone 3-sulfate + H2O | reaction of EC 3.1.6.2 | Pseudomonas aeruginosa | estrone + sulfate | - |
? | |
estrone 3-sulfate + H2O | reaction of EC 3.1.6.2 | Pseudomonas aeruginosa DSM 22644 | estrone + sulfate | - |
? | |
etiocholanolone 3-sulfate + H2O | reaction of EC 3.1.6.2 | Pseudomonas aeruginosa | etiocholanolone + sulfate | - |
? | |
testosterone 17-sulfate + H2O | reaction of EC 3.1.6.2 | Pseudomonas aeruginosa | testosterone + sulfate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AtsA | - |
Pseudomonas aeruginosa |