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Literature summary for 3.1.6.1 extracted from
- Comprehensive kinetic and substrate specificity analysis of an arylsulfatase from Helix pomatia using mass spectrometry (2019), Bioorg. Med. Chem., 27, 955-962 .
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | development of a time-based mass spectrometric assay to analyze substrate conversion and substrate specificity | Helix pomatia |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.112 | - |
methylurolithin A sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 0.131 | - |
resorcinol sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia |  |
| 0.161 | - |
ferulic acid 4-sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 0.175 | - |
4-Methylumbelliferyl sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 0.216 | - |
Indoxyl sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 0.262 | - |
p-coumaric acid sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 0.341 | - |
vanillic acid 4-sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 0.351 | - |
Estrone 3-sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 1.02 | - |
4-ethylphenyl sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 1.4 | - |
p-cresyl sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 1.486 | - |
N-acetyltyramine sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helix pomatia | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-ethylphenyl sulfate + H2O | - |
Helix pomatia | 4-ethylphenol + sulfate | - |
? | |
| 4-methylumbelliferyl sulfate + H2O | - |
Helix pomatia | 4-methylumbelliferone + sulfate | - |
? | |
| estrone 3-sulfate + H2O | - |
Helix pomatia | estrone + sulfate | - |
? | |
| ferulic acid 4-sulfate + H2O | - |
Helix pomatia | ferulic acid + sulfate | - |
? | |
| indoxyl sulfate + H2O | - |
Helix pomatia | indoxol + sulfate | - |
? | |
| methylurolithin A sulfate + H2O | - |
Helix pomatia | methylurolithin A + sulfate | - |
? | |
| N-acetyltyramine sulfate + H2O | - |
Helix pomatia | N-acetyltyramine + sulfate | - |
? | |
| p-coumaric acid sulfate + H2O | - |
Helix pomatia | p-coumaric acid + sulfate | - |
? | |
| p-cresyl sulfate + H2O | - |
Helix pomatia | p-cresol + sulfate | - |
? | |
| resorcinol sulfate + H2O | - |
Helix pomatia | resorcinol + sulfate | - |
? | |
| vanillic acid 4-sulfate + H2O | - |
Helix pomatia | vanillic acid + sulfate | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 92 | - |
resorcinol sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 119 | - |
vanillic acid 4-sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 138 | - |
ferulic acid 4-sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 228 | - |
Indoxyl sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 235 | - |
p-coumaric acid sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 871 | - |
p-cresyl sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 1800 | - |
N-acetyltyramine sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 7150 | - |
4-ethylphenyl sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 28600 | - |
4-Methylumbelliferyl sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 40900 | - |
Estrone 3-sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 88500 | - |
methylurolithin A sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.35 | - |
vanillic acid 4-sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 0.62 | - |
p-cresyl sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 0.69 | - |
resorcinol sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 0.86 | - |
ferulic acid 4-sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 0.9 | - |
p-coumaric acid sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 1.06 | - |
Indoxyl sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 1.2 | - |
N-acetyltyramine sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 7 | - |
4-ethylphenyl sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 117 | - |
Estrone 3-sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 164 | - |
4-Methylumbelliferyl sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia | |
| 787 | - |
methylurolithin A sulfate | pH 7.0, temperature not specified in the publication | Helix pomatia |
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