Literature summary for 3.1.6.1 extracted from

Yuan, M.; Yang, X.; Li, Y.; Liu, H.; Pu, J.; Zhan, C.G.; Liao, F.
Facile alkaline lysis of Escherichia coli cells in high-throughput mode for screening enzyme mutants arylsulfatase as an example (2016), Appl. Biochem. Biotechnol., 179, 545-557 .

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Application

Application	Comment	Organism
analysis	high-throughput screen for mutants expressed in Escherichia coli. The alkaline lysis buffer is 1.0 M Tris-HCl at pH 9.0 plus 0.1 % Tween-20 and 2.0 mM 4-aminobenzamidine, mixed with cell suspension at 8:1 to 12:1 ratio for continuous agitation of mixtures in 96-well plates under room temperature. The enzmye tolerates final 0.1 % Tween-20, and activities in lysates are steady from 3 to 9 h and comparable to sonication treatment but better than freezing-thawing	Pseudomonas aeruginosa

Cloned(Commentary)

Cloned (Comment)	Organism
exprtession in Escherichia coli	Pseudomonas aeruginosa

Protein Variants

Protein Variants	Comment	Organism
M72Q	2fold increase in activity compared with starting mutant G138S	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	-	-	-

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Release 2023.1 (January 2023)