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Literature summary for 3.1.6.1 extracted from

  • Lee, D.; Shin, J.; Jeon, M.; Lee, S.
    Heterologous expression and characterization of a recombinant thermophilic arylsulfatase from Thermotoga maritima (2013), Biotechnol. Bioprocess Eng., 18, 897-902 .
No PubMed abstract available

Application

Application Comment Organism
synthesis the enzyme could be useful for producing a low sulfated agar and electrophoretic grade agarose Thermotoga maritima

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli W3110 with pCol-MICT as the vector Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
SDS-PAGE Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9X229
-
-
Thermotoga maritima DSM 3109 Q9X229
-
-

Purification (Commentary)

Purification (Comment) Organism
partially purified Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl sulfate + H2O
-
Thermotoga maritima 4-nitrophenol + sulfate
-
?
4-nitrophenyl sulfate + H2O
-
Thermotoga maritima DSM 3109 4-nitrophenol + sulfate
-
?
agar + H2O sulfate content of agar is diminished to 40% after a 12 h treatment Thermotoga maritima ?
-
?
agar + H2O sulfate content of agar is diminished to 40% after a 12 h treatment Thermotoga maritima DSM 3109 ?
-
?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
Thermotoga maritima

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 90 60°C: about 60% of maximal activity, 90°C: about 30% of maximal activity Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Thermotoga maritima

pH Range

pH Minimum pH Maximum Comment Organism
6 8 pH 6.0: about 25% of maximal activity, pH 8.0: about 85% of maximal activity Thermotoga maritima