Application | Comment | Organism |
---|---|---|
synthesis | the enzyme could be useful for producing a low sulfated agar and electrophoretic grade agarose | Thermotoga maritima |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli W3110 with pCol-MICT as the vector | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
SDS-PAGE | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | Q9X229 | - |
- |
Thermotoga maritima DSM 3109 | Q9X229 | - |
- |
Purification (Comment) | Organism |
---|---|
partially purified | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl sulfate + H2O | - |
Thermotoga maritima | 4-nitrophenol + sulfate | - |
? | |
4-nitrophenyl sulfate + H2O | - |
Thermotoga maritima DSM 3109 | 4-nitrophenol + sulfate | - |
? | |
agar + H2O | sulfate content of agar is diminished to 40% after a 12 h treatment | Thermotoga maritima | ? | - |
? | |
agar + H2O | sulfate content of agar is diminished to 40% after a 12 h treatment | Thermotoga maritima DSM 3109 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 65000, SDS-PAGE | Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Thermotoga maritima |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 90 | 60°C: about 60% of maximal activity, 90°C: about 30% of maximal activity | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Thermotoga maritima |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | pH 6.0: about 25% of maximal activity, pH 8.0: about 85% of maximal activity | Thermotoga maritima |