Literature summary for 3.1.6.1 extracted from

Kim, D.E.; Kim, K.H.; Bae, Y.J.; Lee, J.H.; Jang, Y.H.; Nam, S.W.
Purification and characterization of the recombinant arylsulfatase cloned from Pseudoalteromonas carrageenovora (2005), Protein Expr. Purif., 39, 107-115.

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Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Escherichia coli BL21	Pseudoalteromonas carrageenovora

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	0.1 mM EDTA 46.8% relative activity, 0.5 mM EDTA 39.6% relative activity and 1.0 mM EDTA 30.5% relative activity compared to the control	Pseudoalteromonas carrageenovora
PMSF	0.1 mM PMSF 92.7% relative activity, 0.5 mM PMSF 82.6% relative activity and 1.0 mM PMSF 73.5% relative activity compared to the control	Pseudoalteromonas carrageenovora

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.67	-	p-Nitrophenyl sulfate	6.5 nM purified arylsulfatase incubated at 45°C and at pH 7.0 in the presence of 10 mM sulfate	Pseudoalteromonas carrageenovora
1.15	-	p-Nitrophenyl sulfate	6.5 nM purified arylsulfatase incubated at 45°C and at pH 7.0	Pseudoalteromonas carrageenovora

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
33000	-	SDS-PAGE	Pseudoalteromonas carrageenovora

Organism

Organism	UniProt	Comment	Textmining
Pseudoalteromonas carrageenovora	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
DEAE-cellulose anion-exchange and Heparin-Sepharose CL 6B affinity chromatography, all steps at 4°C	Pseudoalteromonas carrageenovora

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
468	-	7.1 mM p-nitrophenyl sulfate at 45°C and at pH 8.5, after Heparin-Sepharose column chromatography	Pseudoalteromonas carrageenovora

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
p-nitrophenyl sulfate + H2O	-	Pseudoalteromonas carrageenovora	p-nitrophenol + sulfate	-	?

Synonyms

Synonyms	Comment	Organism
arylsulfatase	-	Pseudoalteromonas carrageenovora

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
45	-	purified arylsulfatase analysed at pH 7.0 and temperature between 10 to 80°C, p-nitrophenyl sulfate as substrate	Pseudoalteromonas carrageenovora

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1000	-	p-Nitrophenyl sulfate	6.5 nM purified arylsulfatase, at 45°C and pH 7.0	Pseudoalteromonas carrageenovora

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	activity of purified arylsulfatase analysed at 40°C and at pH 4.5 to 10, p-nitrophenyl sulfate as substrate	Pseudoalteromonas carrageenovora

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Release 2023.1 (January 2023)