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Literature summary for 3.1.6.1 extracted from

  • Sampson, E.J.; Vergara, E.V.; Fedor, J.M.; Funk, M.O.; Benkovic, S.J.
    On the physical properties and mechanism of action of arylsulfate sulfohydrolase II from Aspergillus oryzae (1975), Arch. Biochem. Biophys., 169, 372-383.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
4-nitrophenyl phosphate hydrolysis of 4-nitrophenyl sulfate Aspergillus oryzae
Estrone 3-sulfate
-
Aspergillus oryzae
N-benzyloxycarbonyl-L-Phe-L-Tyr-O-sulfate methyl ester hydrolysis of 4-nitrophenyl sulfate Aspergillus oryzae
Phenolphthalein disulfate hydrolysis of 4-nitrophenyl sulfate Aspergillus oryzae
SO42- competitive Aspergillus oryzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.344
-
Estrone 3-sulfate
-
Aspergillus oryzae
1.05
-
N-benzyloxycarbonyl-L-Phe-L-Tyr-O-sulfate
-
Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
2 * 40000, SDS-PAGE Aspergillus oryzae
94900
-
sedimentation equilibrium Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-carboxyphenyl sulfate + H2O
-
Aspergillus oryzae 2-carboxyphenol + sulfate
-
?
2-chloro-4-nitrophenyl sulfate + H2O
-
Aspergillus oryzae sulfate + 2-chloro-4-nitrophenol
-
?
2-nitrophenyl sulfate + H2O
-
Aspergillus oryzae 2-nitrophenol + sulfate
-
?
4-nitrophenyl sulfate + H2O
-
Aspergillus oryzae 4-nitrophenol + sulfate
-
?
benzyloxycarbonyl-L-Phe-L-Tyr-O-sulfate methyl ester + H2O
-
Aspergillus oryzae benzyloxycarbonyl-L-Phe-L-Tyr + methylsulfate
-
?
estrone 3-sulfate + H2O
-
Aspergillus oryzae sulfate + estrone
-
?
N-benzyloxycarbonyl-L-Phe-L-Tyr-O-sulfate + H2O
-
Aspergillus oryzae N-benzyloxycarbonyl-L-Phe-L-Tyr + -sulfate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 40000, SDS-PAGE Aspergillus oryzae

Synonyms

Synonyms Comment Organism
arylsulfate sulfohydrolase II
-
Aspergillus oryzae