Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.5.B1 extracted from

  • Mauney, C.H.; Perrino, F.W.; Hollis, T.
    Identification of inhibitors of the dNTP triphosphohydrolase SAMHD1 using a novel and direct high-throughput assay (2018), Biochemistry, 57, 6624-6636 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
analysis high-throughput screen that directly measures SAMHD1 catalytic activity, utilizing bis(4-nitrophenyl) phosphate as the substrate and Mn2+ as the activating cation Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
amrinone IC50 value below 250 microM, Hill coefficient between 0.5 and 1.5 Homo sapiens
ergotamine IC50 value below 250 microM, Hill coefficient between 0.5 and 1.5 Homo sapiens
Hexestrol IC50 value below 250 microM, Hill coefficient between 0.5 and 1.5 Homo sapiens
L-thyroxine IC50 value below 250 microM, Hill coefficient between 0.5 and 1.5 Homo sapiens
lomofungin IC50 value below 250 microM, Hill coefficient between 0.5 and 1.5 Homo sapiens
montelukast IC50 value below 250 microM, Hill coefficient between 0.5 and 1.5 Homo sapiens
retinoic acid IC50 value below 250 microM, Hill coefficient between 0.5 and 1.5 Homo sapiens
sulindac IC50 value below 250 microM, Hill coefficient between 0.5 and 1.5 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ with substrate bis(4-nitrophenyl) phosphate, enzyme is able to tetramerize in presence of Mn2+ Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9Y3Z3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bis(4-nitrophenyl) phosphate + H2O
-
Homo sapiens 4-nitrophenyl phosphate + 4-nitrophenol
-
?