Literature summary for 3.1.5.B1 extracted from

Mauney, C.H.; Rogers, L.C.; Harris, R.S.; Daniel, L.W.; Devarie-Baez, N.O.; Wu, H.; Furdui, C.M.; Poole, L.B.; Perrino, F.W.; Hollis, T.
The SAMHD1 dNTP triphosphohydrolase is controlled by a redox switch (2017), Antioxid. Redox Signal., 27, 1317-1331 .

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Protein Variants

Protein Variants	Comment	Organism
C320A	mutant shows the formation of disulfide bonds similar to wild type	Homo sapiens
C341A	mutant shows no disulfide species	Homo sapiens
C350A	mutant shows no disulfide species	Homo sapiens
C522A	mutant shows no significant inhibition by oxidation	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
dimedone	oxidization in the presence of dimedone results in multiple cysteine residues displaying formation of sulfenic acid. Cys341-Cys350 is the predominant intrachain disulfide bond formed	Homo sapiens
H2O2	90% inhibition at 0.1 mM. dNTPase activity of SAMHD1 is reversibly inhibited by oxidation, addition of dithiothreitol restores the activity. Oxidation prevents subunit oligomerization	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	oxidation of the protein occurs in the cytoplasm	Homo sapiens	5737	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
72200	-	dynamic light scattering, absence of activating nucleotides	Homo sapiens
142000	-	dynamic light scattering, oxidized enzyme, presence of activating nucleotides	Homo sapiens
292000	-	dynamic light scattering, presence of activating nucleotides	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q9Y3Z3	-	-

Subunits

Subunits	Comment	Organism
dimer	2 * 72200, calculated from sequence, presence of activating nucleotides, oxidized enzyme	Homo sapiens
monomer	1 * 72200, calculated from sequence, absence of activating nucleotides	Homo sapiens
tetramer	4 * 72200, calculated from sequence, presence of activating nucleotides	Homo sapiens

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Release 2023.1 (January 2023)