Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dGTP | allosteric activator. Allosteric dGTP binding induces conformational changes at the active site, allowing a more stable interaction with the substrate and explaining the dGTP-induced isoform SAMHD1 dNTPase activity. Mutations of dGTP binding residues in the allosteric site affect tetramer formation, dNTPase activity and HIV-1 restriction | Homo sapiens | |
additional information | dGTP, but not the other dNTPs, is required for the formation and maintenance of the catalytically more active SAMHD1 tetramer | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
in complex with the allosteric activator and substrate dGTP/dATP, to 1.8 A resolution | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D137A | reduced ability to form tetramers, reduced dNTPase activity | Homo sapiens |
Q142E/R145K | reduced ability to form tetramers, reduced dNTPase activity | Homo sapiens |
R333E | reduced ability to form tetramers, reduced dNTPase activity | Homo sapiens |
R333E/R451E | reduced ability to form tetramers, reduced dNTPase activity | Homo sapiens |
R451E | reduced ability to form tetramers, reduced dNTPase activity | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
and 280000, gel filtration | Homo sapiens |
72600 | - |
1 * 72600, and tetramer, 4 * 72600, analytical ultracentrifugation. Formation of tetramer is induced by dGTP, tetramer is a more potent dNTPase than monomer | Homo sapiens |
72600 | - |
4 * 72600, and monomer, 1 * 72600, analytical ultracentrifugation. Formation of tetramer is induced by dGTP, tetramer is a more potent dNTPase than monomer | Homo sapiens |
280000 | - |
and 72000, gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dATP + H2O | - |
Homo sapiens | deoxyadenosine + triphosphate | - |
? | |
dGTP + H2O | - |
Homo sapiens | deoxyguanosine + triphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 72600, and tetramer, 4 * 72600, analytical ultracentrifugation. Formation of tetramer is induced by dGTP, tetramer is a more potent dNTPase than monomer | Homo sapiens |
tetramer | 4 * 72600, and monomer, 1 * 72600, analytical ultracentrifugation. Formation of tetramer is induced by dGTP, tetramer is a more potent dNTPase than monomer | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
deoxynucleoside triphosphate triphosphohydrolase | - |
Homo sapiens |
SAMHD1 | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | allosteric dGTP binding induces conformational changes at the active site, allowing a more stable interaction with the substrate and explaining the dGTP-induced dNTPase activity. Mutations of dGTP binding residues in the allosteric site affect tetramer formation, dNTPase activity and HIV-1 restriction. dGTP-triggered tetramer formation is also important for SAMHD1-mediated retrotransposition of LINE-1 elements | Homo sapiens |