Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dGTP | low concentrations of either GTP or dGTP stimulate the reaction maximally, Km value for binding to allosteric site 1 is 0.00015 mM with substrate dATP | Homo sapiens | |
dGTP | low concentrations of either GTP or dGTP stimulate the reaction maximally, Km value for binding to allosteric site 1 is 0.0008 mM with substrate dATP | Mus musculus | |
GTP | low concentrations of either GTP or dGTP stimulate the reaction maximally, Km value for binding to allosteric site 1 is 0.00015 mM with substrate dATP. In quiescent cells where the enzyme is maximally expressed GTP binds to allosteric site 1 with very high affinity, stabilizing site 2 of the tetrameric structure. Any canonical dNTP can bind to site 2 and activate the enzyme, but in cells only dATP or dTTP are present at sufficient concentrations. Tetrameric enzyme is activated for the hydrolysis of any dNTP only after binding of a dNTP to site 2 | Homo sapiens | |
GTP | low concentrations of either GTP or dGTP stimulate the reaction maximally, Km value for binding to allosteric site 1 is 0.0005 mM with substrate dATP. In quiescent cells where the enzyme is maximally expressed GTP binds to allosteric site 1 with very high affinity, stabilizing site 2 of the tetrameric structure. Any canonical dNTP can bind to site 2 and activate the enzyme, but in cells only dATP or dTTP are present at sufficient concentrations. Tetrameric enzyme is activated for the hydrolysis of any dNTP only after binding of a dNTP to site 2 | Mus musculus | |
additional information | in quiescent cells where the enzyme is maximally expressed GTP binds to allosteric site 1 with very high affinity, stabilizing site 2 of the tetrameric structure. Any canonical dNTP can bind to site 2 and activate the enzyme, but in cells only dATP or dTTP are present at sufficient concentrations. Tetrameric enzyme is activated for the hydrolysis of any dNTP only after binding of a dNTP to site 2 | Homo sapiens | |
additional information | in quiescent cells where the enzyme is maximally expressed GTP binds to allosteric site 1 with very high affinity, stabilizing site 2 of the tetrameric structure. Any canonical dNTP can bind to site 2 and activate the enzyme, but in cells only dATP or dTTP are present at sufficient concentrations. Tetrameric enzyme is activated for the hydrolysis of any dNTP only after binding of a dNTP to site 2 | Mus musculus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
expression in Escherichia coli | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
dCTP | pH 7.5, 37°C, presence of 0.02 mM GTP plus 0.02 mM dATP | Mus musculus | |
0.22 | - |
dGTP | pH 7.5, 37°C | Mus musculus | |
0.22 | - |
dATP | pH 7.5, 37°C, presence of 0.02 mM GTP | Mus musculus | |
0.24 | - |
dGTP | pH 7.5, 37°C | Homo sapiens | |
0.28 | - |
dTTP | pH 7.5, 37°C, presence of 0.02 mM GTP plus 0.02 mM dATP | Mus musculus | |
0.29 | - |
dCTP | pH 7.5, 37°C, presence of 0.02 mM GTP plus 0.02 mM dATP | Homo sapiens | |
0.3 | - |
dTTP | pH 7.5, 37°C, presence of 0.02 mM GTP plus 0.02 mM dATP | Homo sapiens | |
0.34 | - |
dATP | pH 7.5, 37°C, presence of 0.02 mM GTP | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9Y3Z3 | - |
- |
Mus musculus | Q60710 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dATP + H2O | - |
Homo sapiens | deoxyadenosine + triphosphate | - |
? | |
dATP + H2O | - |
Mus musculus | deoxyadenosine + triphosphate | - |
? | |
dCTP + H2O | - |
Homo sapiens | deoxycytidine + triphosphate | - |
? | |
dCTP + H2O | - |
Mus musculus | deoxycytidine + triphosphate | - |
? | |
dGTP + H2O | - |
Homo sapiens | deoxyguanosine + triphosphate | - |
? | |
dGTP + H2O | - |
Mus musculus | deoxyguanosine + triphosphate | - |
? | |
dTTP + H2O | - |
Homo sapiens | deoxythymidine + triphosphate | - |
? | |
dTTP + H2O | - |
Mus musculus | deoxythymidine + triphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
deoxyribonucleotide triphosphohydrolase | - |
Homo sapiens |
deoxyribonucleotide triphosphohydrolase | - |
Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
dGTP | pH 7.5, 37°C | Homo sapiens | |
0.9 | - |
dCTP | pH 7.5, 37°C, presence of 0.02 mM GTP plus 0.02 mM dATP | Homo sapiens | |
1 | - |
dATP | pH 7.5, 37°C, presence of 0.02 mM GTP | Homo sapiens | |
1 | - |
dTTP | pH 7.5, 37°C, presence of 0.02 mM GTP plus 0.02 mM dATP | Homo sapiens | |
2.2 | - |
dCTP | pH 7.5, 37°C, presence of 0.02 mM GTP plus 0.02 mM dATP | Mus musculus | |
2.9 | - |
dGTP | pH 7.5, 37°C | Mus musculus | |
4.1 | - |
dTTP | pH 7.5, 37°C, presence of 0.02 mM GTP plus 0.02 mM dATP | Mus musculus | |
4.4 | - |
dATP | pH 7.5, 37°C, presence of 0.02 mM GTP | Mus musculus |
Organism | Comment | Expression |
---|---|---|
Mus musculus | maximum expression in quiescent cells | additional information |
Homo sapiens | maximum expressionin quescent cells | additional information |