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Literature summary for 3.1.4.54 extracted from

  • Wang, J.; Okamoto, Y.; Tsuboi, K.; Ueda, N.
    The stimulatory effect of phosphatidylethanolamine on N-acylphosphatidylethanolamine-hydrolyzing phospholipase D (NAPE-PLD) (2007), Neuropharmacology, 54, 8-15.
    View publication on PubMed
Search for more literature for 3.1.4.54

Activating Compound

Activating Compound Comment Organism Structure
1,2-dioleoylphosphatidylcholine 0.1 mM, 1.3fold stimulation Rattus norvegicus
1,2-dioleoylphosphatidylethanolamine 0.1 mM, increases activity with N-palmitoylphosphatidylamine (0.025 mM) 3.3fold, increases activity with N-arachidonoylphosphatidylamine (0.025 mM), N-oleoylphosphatidylamine (0.025 mM) and N-stearoylphosphatidylamine (0.025 mM), 2.6-2.7fold Rattus norvegicus
1,2-dipalmitoylphosphatidylcholine 0.1 mM, 1.3fold stimulation Rattus norvegicus
1,2-dipalmitoylphosphatidylethanolamine
-
 Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 10 mM, more than 90% inhibition Rattus norvegicus
Cu2+ 10 mM, more than 90% inhibition Rattus norvegicus
Hg2+ 10 mM, more than 90% inhibition Rattus norvegicus
Zn2+ 10 mM, more than 90% inhibition Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0017
-
 N-palmitoylphosphatidylethanolamine pH 7.4, 37°C, 10 mM CaCl2, 0.1 mM 1,2-dioleoyl-phosphatidylethanolamine Rattus norvegicus
0.0022
-
 N-palmitoylphosphatidylethanolamine pH 7.4, 37°C, 5 mM EDTA Rattus norvegicus
0.0038
-
 N-palmitoylphosphatidylethanolamine pH 7.4, 37°C, 5 mM EDTA, 0.1 mM 1,2-dipalmitoyl-phosphatidylcholine Rattus norvegicus
0.004
-
 N-palmitoylphosphatidylethanolamine pH 7.4, 37°C, 10 mM CaCl2 Rattus norvegicus
0.0069
-
 N-palmitoylphosphatidylethanolamine pH 7.4, 37°C, 10 mM CaCl2, 0.1 mM 1,2-dioleoyl-phosphatidylserine Rattus norvegicus
0.0116
-
 N-palmitoylphosphatidylethanolamine pH 7.4, 37°C, 5 mM EDTA, 0.1 mM 1,2-dioleoyl-phosphatidylethanolamine Rattus norvegicus
0.0122
-
 N-palmitoylphosphatidylethanolamine pH 7.4, 37°C, 10 mM CaCl2, 0.1 mM 1,2-dipalmitoyl-phosphatidylcholine Rattus norvegicus
0.0122
-
 N-palmitoylphosphatidylethanolamine pH 7.4, 37°C, 5 mM EDTA, 0.1 mM 1,2-dioleoyl-phosphatidylserine Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Rattus norvegicus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 10 mM, stimulates Rattus norvegicus
Ca2+ in the presence of 100 mM phospholipid (phosphatidylethanolamine, phosphatidylcholine, or phosphatidylserine), Ca2+ dose-dependently activates NAPE-PLD in varied degrees. Ca2+ at 10 mM stimulates the activity 2.1 fold in the presence of phosphatidylethanolamine. In the presence of phosphatidylcholine or phosphatidylserine, the stimulatory effects of 10 mM Ca2+ (5.2 and 10 fold, respectively) are similar to the effect by 10 mM Ca2+ in the absence of phospholipids (7fold) Rattus norvegicus
Co2+ 10 mM, stimulates Rattus norvegicus
Mg2+ 10 mM, stimulates Rattus norvegicus
Ni2+ 10 mM, stimulates Rattus norvegicus
Sr2+ 10 mM, stimulates Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus Q769K2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-arachidonoylphosphatidylethanolamine + H2O
-
 Rattus norvegicus N-arachidonoylethanolamine + phosphatidate
-
 ?
N-oleoylphosphatidylethanolamine + H2O
-
 Rattus norvegicus N-oleoylethanolamine + phosphatidate
-
 ?
N-palmitoylphosphatidylethanolamine + H2O
-
 Rattus norvegicus N-palmitoylethanolamine + phosphatidate
-
 ?
N-stearoylphosphatidylethanolamine + H2O
-
 Rattus norvegicus N-stearoylethanolamine + phosphatidate
-
 ?

Synonyms

Synonyms Comment Organism
NAPE-PLD
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Rattus norvegicus