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Literature summary for 3.1.4.54 extracted from
- N-acylphosphatidylethanolamine-hydrolyzing phospholipase D: a novel enzyme of the beta-lactamase fold family releasing anandamide and other N-acylethanolamines (2005), Life Sci., 77, 1750-1758.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| spermine | - |
Rattus norvegicus |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in COS-7 cells | Mus musculus |
| overexpression in COS-7 cells | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
N-palmitoylphosphatidylethanolamine | 37°C, pH not specified in the publication, Ca2+ stimulated enzyme | Rattus norvegicus | |
| 0.045 | - |
N-palmitoylphosphatidylethanolamine | 37°C, pH not specified in the publication, Triton X-100 stimulated enzyme | Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | stimulated up to 30fold by millimolar order of Ca2+. This effect is fully substituted by other divalent cations such as Co2+, Mg2+, Mn2+, Ba2+, Sr2+ and Ni2+ | Rattus norvegicus | |
| Ca2+ | stimulated up to 30fold by millimolar order of Ca2+. This effect is fully substituted by other divalent cations such as Co2+, Mg2+, Mn2+, Ba2+, Sr2+ and Ni2+ | Rattus norvegicus | |
| Co2+ | stimulated up to 30fold by millimolar order of Ca2+. This effect is fully substituted by other divalent cations such as Co2+, Mg2+, Mn2+, Ba2+, Sr2+ and Ni2+ | Rattus norvegicus | |
| Mg2+ | stimulated up to 30fold by millimolar order of Ca2+. This effect is fully substituted by other divalent cations such as Co2+, Mg2+, Mn2+, Ba2+, Sr2+ and Ni2+ | Rattus norvegicus | |
| Mn2+ | stimulated up to 30fold by millimolar order of Ca2+. This effect is fully substituted by other divalent cations such as Co2+, Mg2+, Mn2+, Ba2+, Sr2+ and Ni2+ | Rattus norvegicus | |
| Ni2+ | stimulated up to 30fold by millimolar order of Ca2+. This effect is fully substituted by other divalent cations such as Co2+, Mg2+, Mn2+, Ba2+, Sr2+ and Ni2+ | Rattus norvegicus | |
| Sr2+ | stimulated up to 30fold by millimolar order of Ca2+. This effect is fully substituted by other divalent cations such as Co2+, Mg2+, Mn2+, Ba2+, Sr2+ and Ni2+ | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
x * 46000, SDS-PAGE | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-arachidonoylphosphatidylethanolamine + H2O | Mus musculus | the enzyme is involved in the biosynthesis of anandamide, an endocannabinoid that belongs to the class of bioactive, long-chain N-acylethanolamines | N-arachidonoylethanolamine + phosphatidic acid | N-arachidonoylethanolamine i.e. anandamide | ? | |
| N-arachidonoylphosphatidylethanolamine + H2O | Rattus norvegicus | the enzyme is involved in the biosynthesis of anandamide, an endocannabinoid that belongs to the class of bioactive, long-chain N-acylethanolamines | N-arachidonoylethanolamine + phosphatidic acid | N-arachidonoylethanolamine i.e. anandamide | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | Q8BH82 | - |
- |
| Rattus norvegicus | Q769K2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | widely distributed with higher specific activities in brain, kidney and testis | Mus musculus | - |
| heart | - |
Rattus norvegicus | - |
| kidney | widely distributed with higher specific activities in brain, kidney and testis | Mus musculus | - |
| testis | widely distributed with higher specific activities in brain, kidney and testis | Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00002 | - |
the endogenous NAPE-PLD activity with the homogenates of COS-7 cells transfected with the insert-free vector, pH and temperature not specified in the publication | Mus musculus |
| 0.019 | - |
hydrolysis of N-palmitoylphosphatidylethanolamine, homogenates of transfected cells, pH and temperature not specified in the publication | Mus musculus |
| 0.406 | - |
37°C, pH and temperature not specified in the publication | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme hydrolyzes N-acyl-2-lysophosphatidylamines at a much lower rate than N-acyl-phosphatidylethanolamines | Mus musculus | ? | - |
? | |
| N-arachidonoylphosphatidylethanolamine + H2O | the enzyme is involved in the biosynthesis of anandamide, an endocannabinoid that belongs to the class of bioactive, long-chain N-acylethanolamines | Mus musculus | N-arachidonoylethanolamine + phosphatidic acid | N-arachidonoylethanolamine i.e. anandamide | ? | |
| N-arachidonoylphosphatidylethanolamine + H2O | the enzyme is involved in the biosynthesis of anandamide, an endocannabinoid that belongs to the class of bioactive, long-chain N-acylethanolamines | Rattus norvegicus | N-arachidonoylethanolamine + phosphatidic acid | N-arachidonoylethanolamine i.e. anandamide | ? | |
| N-arachidonoylphosphatidylethanolamine + H2O | Km values and Vmax values for N-palmitoylphosphatidylethanolamine, N-arachidonoylphosphatidylethanolamine, N-oleoylphosphatidylethanolamine, and N-stearoylphosphatidylethanolamine are similar. The enzyme does not have selectivity for N-acyl groups of N-acylphosphatidylamines | Mus musculus | N-arachidonoylethanolamine + phosphatidate | - |
? | |
| N-oleoylphosphatidylethanolamine + H2O | Km values and Vmax values for N-palmitoylphosphatidylethanolamine, N-arachidonoylphosphatidylethanolamine, N-oleoylphosphatidylethanolamine, and N-stearoylphosphatidylethanolamine are similar. The enzyme does not have selectivity for N-acyl groups of N-acylphosphatidylamines | Mus musculus | N-oleoylethanolamine + phosphatidate | - |
? | |
| N-palmitoylphosphatidylethanolamine + H2O | - |
Rattus norvegicus | N-palmitoylethanolamine + phosphatidate | - |
? | |
| N-palmitoylphosphatidylethanolamine + H2O | Km values and Vmax values for N-palmitoyl-phosphatidylethanolamine, N-arachidonoyl-phosphatidylethanolamine, N-oleoyl-phosphatidylethanolamine, and N-stearoyl-phosphatidylethanolamine are similar. The enzyme does not have selectivity for N-acyl groups of N-acylphosphatidylamines | Mus musculus | N-palmitoylethanolamine + phosphatidate | - |
? | |
| N-stearoylphosphatidylethanolamine + H2O | Km values and Vmax values for N-palmitoylphosphatidylethanolamine, N-arachidonoylphosphatidylethanolamine, N-oleoylphosphatidylethanolamine, and N-stearoylphosphatidylethanolamine are similar. The enzyme does not have selectivity for N-acyl groups of N-acylphosphatidylamines | Mus musculus | N-stearoylethanolamine + phosphatidate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 46000, SDS-PAGE | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NAPE-PLD | - |
Mus musculus |
| NAPE-PLD | - |
Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Rattus norvegicus |
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Release 2023.1 (January 2023)
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