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Literature summary for 3.1.4.53 extracted from

  • Ramanujam, R.; Naqvi, N.
    PdeH, a high-affinity cAMP phosphodiesterase, is a key regulator of asexual and pathogenic differentiation in Magnaporthe oryzae (2010), PLoS Pathog., 6, e1000897.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.53

Protein Variants

Protein Variants Comment Organism
additional information generation of gene-deletion mutants of the high-affinity PdeH cAMP phosphodiesterase, phenotypes, overview Pyricularia oryzae
additional information generation of gene-deletion mutants of the low-affinity PdeL cAMP phosphodiesterase, phenotypes, overview Pyricularia oryzae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol a fully functional GFP-tagged PdeH is cytosolic but associates dynamically with the plasma membrane and vesicular compartments Pyricularia oryzae 5829
-
additional information the deletion mutant the GFP-PdeL localizes predominantly to the nucleus Pyricularia oryzae
-
-

Organism

Organism UniProt Comment Textmining
Pyricularia oryzae D0ERY7 PdeH; enzymes PDEH and PDEL
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Pyricularia oryzae D0ERY8 PdeL; enzyme PDEL
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine 3',5'-cyclic phosphate + H2O
-
 Pyricularia oryzae adenosine 5'-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
cAMP phosphodiesterase
-
 Pyricularia oryzae
PdeH
-
 Pyricularia oryzae
PdeL
-
 Pyricularia oryzae

General Information

General Information Comment Organism
malfunction a pdeHDELTA/pdeLDELTA mutant shows reduced conidiation, exhibits dramatically increased cAMP levels relative to the wild-type, and is completely defective in virulence Pyricularia oryzae
malfunction loss of PdeH leads to increased accumulation of intracellular cAMP during vegetative and infectious growth. Furthermore, the pdeHD shows 2-3fold enhanced conidiation, precocious appressorial development, loss of surface dependency during pathogenesis, and highly reduced in planta growth and host colonization. A pdeHDELTA/pdeLDELTA mutant shows reduced conidiation, exhibits dramatically increased cAMP levels relative to the wild-type, and is completely defective in virulence Pyricularia oryzae
metabolism cyclic AMP-dependent pathways mediate the communication between external stimuli and the intracellular signaling machinery, thereby influencing important aspects of cellular growth, morphogenesis and differentiation. Crucial to proper function and robustness of these signaling cascades is the strict regulation and maintenance of intracellular levels of cAMP through a fine balance between biosynthesis, by adenylate cyclases, and hydrolysis, by cAMP phosphodiesterases Pyricularia oryzae
physiological function expendable PdeL function Pyricularia oryzae
physiological function PDEH is necessary for proper aerial hyphal growth. PdeH-dependent biphasic regulation of cAMP levels during early and late stages of appressorial development. PdeH-mediated sustainance and dynamic regulation of cAMP signaling during Magnaporthe oryzae development is crucial for successful establishment and spread of the blast disease in rice. The PdeH activity is a key regulator of asexual and pathogenic development in Magnaporthe oryzae Pyricularia oryzae