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Literature summary for 3.1.4.53 extracted from

  • Diaz-Benjumea, R.; Laxman, S.; Hinds, T.R.; Beavo, J.A.; Rascon, A.
    Characterization of a novel cAMP-binding, cAMP-specific cyclic nucleotide phosphodiesterase (TcrPDEB1) from Trypanosoma cruzi (2006), Biochem. J., 399, 305-314.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
cAMP binds to N-terminus and GAF domain, affinity of 0.0005 mM Trypanosoma cruzi
cAMP binds to N-terminus and GAF domain, affinity of 0.001 mM Trypanosoma cruzi
cGMP binds to N-terminus and GAF domain, affinity of 0.003 mM Trypanosoma cruzi
additional information enzyme does not bind cGMP Trypanosoma cruzi

Cloned(Commentary)

Cloned (Comment) Organism
expression of TcrPDEB1 catalytic domain in Escherichia coli Trypanosoma cruzi

Inhibitors

Inhibitors Comment Organism Structure
dipyridamole
-
Trypanosoma cruzi
EHNA
-
Trypanosoma cruzi
erythro-9-(2-hydroxy-3-nonyl)adenine i.e. EHNA Trypanosoma cruzi
N-[(5S)-5-[2-(3-fluorophenyl)acetamido]-6-[4-(6-methylpyridin-2-yl)piperazin-1-yl]-6-oxohexyl]prop-2-enamide
-
Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0112
-
adenosine 3',5'-cyclic phosphate pH 7.5, 30°C Trypanosoma cruzi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
102500
-
x * 102500, calculated Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi
-
isoform PDEB2, expression in all developmental stages
-
Trypanosoma cruzi Q49UB9
-
-
Trypanosoma cruzi Q49UB9 isoform PDEB1, expression in all developmental stages
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-O-(N-methylanthraniloyl)adenosine-3',5'-cyclic monophosphate + H2O fluorescent cAMP analogue Trypanosoma cruzi 2'-O-(N-methylanthraniloyl)adenosine-5'-monophosphate
-
?
adenosine 3',5'-cyclic phosphate + H2O
-
Trypanosoma cruzi adenosine 5'-phosphate
-
?
cAMP + H2O
-
Trypanosoma cruzi 5'-AMP
-
?
additional information no activity with up to 0.1 mM cGMP Trypanosoma cruzi ?
-
?

Subunits

Subunits Comment Organism
? x * 102500, calculated Trypanosoma cruzi
More enzyme contains two GAF domains and a catalytic domain highly homologous with that of the Trypanosoma brucei family Trypanosoma cruzi

Synonyms

Synonyms Comment Organism
TcrPDEB1
-
Trypanosoma cruzi

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00113
-
pH 7.5, 30°C Trypanosoma cruzi dipyridamole
0.0113
-
30°C, pH 7.5 Trypanosoma cruzi dipyridamole
0.015
-
30°C, pH 7.5 Trypanosoma cruzi dipyridamole
0.105
-
30°C, pH 7.5 Trypanosoma cruzi N-[(5S)-5-[2-(3-fluorophenyl)acetamido]-6-[4-(6-methylpyridin-2-yl)piperazin-1-yl]-6-oxohexyl]prop-2-enamide
0.128
-
30°C, pH 7.5 Trypanosoma cruzi EHNA