Cloned (Comment) | Organism |
---|---|
TbPDE1 is expressed in Saccharomyces cerevisiae at levels that are sufficient to produce a clear phenotype (heat-shock resistance, growth as a smooth suspension), but that are too low to be detectable in PDE assay of cell lysates. The N-terminally truncated construct pET-PDE1-(Arg189-Thr620) is expressed in Escherichia coli as an active enzyme | Trypanosoma brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dipyridamole | IC50: 0.013 mM | Trypanosoma brucei | |
etazolate | IC50: 0.025 mM | Trypanosoma brucei | |
ethaverine | IC50: 0.008 mM | Trypanosoma brucei | |
papaverine | IC50: 0.03 mM | Trypanosoma brucei | |
rolipram | IC50: 0.28 mM | Trypanosoma brucei | |
sildenafil | IC50: 0.001 mM | Trypanosoma brucei | |
trequinsin | IC50: 0.0025 mM | Trypanosoma brucei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bloodstream form | - |
Trypanosoma brucei | - |
procyclic form | - |
Trypanosoma brucei | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3',5'-cAMP + H2O | specific for cAMP | Trypanosoma brucei | 5'-AMP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TbPDE1 | - |
Trypanosoma brucei |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.001 | - |
IC50: 0.001 mM | Trypanosoma brucei | sildenafil | |
0.0025 | - |
IC50: 0.0025 mM | Trypanosoma brucei | trequinsin | |
0.008 | - |
IC50: 0.008 mM | Trypanosoma brucei | ethaverine | |
0.013 | - |
IC50: 0.013 mM | Trypanosoma brucei | dipyridamole | |
0.025 | - |
IC50: 0.025 mM | Trypanosoma brucei | etazolate | |
0.03 | - |
IC50: 0.03 mM | Trypanosoma brucei | papaverine | |
0.28 | - |
IC50: 0.28 mM | Trypanosoma brucei | rolipram |