Literature summary for 3.1.4.52 extracted from

Gao, X.; Dong, X.; Subramanian, S.; Matthews, P.M.; Cooper, C.A.; Kearns, D.B.; Dann, C.E.
Engineering of Bacillus subtilis strains to allow rapid characterization of heterologous diguanylate cyclases and phosphodiesterases (2014), Appl. Environ. Microbiol., 80, 6167-6174.

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Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of genetic constructs in Bacillus subtilis strain DS2569 to generate phage lysates for transduction	Clostridioides difficile

Protein Variants

Protein Variants	Comment	Organism
additional information	system development to survey the activity of putative c-di-GMP metabolic enzymes, method design and evaluation, overview. Generation of 19 inducible translational fusion constructs for genes encoding putative c-di-GMP phosphodiesterases from Clostridium difficile 630 in engineered Clostridium difficile strain NPS235. To construct a c-di-GMP-responsive biosensor, a chimeric ribo switch is engineered upstream of the coding sequence for green fluorescent protein with nucleotides -564 to-86 of Bacillus cereus gene bc_4140 of strain ATCC 14579, containing an M-box riboswitch promoter, aptamer, transcriptional terminator, and flanking sequences, as a scaffold, construction of c-di-GMP riboswitch reporter strains using main parts of the sequence from Bacillus cereus strain ATCC 14579, gene bc_4140, UniProt ID Q818V1 and the aptamer sequence from a c-di-GMP-responsive riboswitch (GEMM motif), of Bacillus cereus gene bce_0489, strain ATCC 10987	Clostridioides difficile

Organism

Organism	UniProt	Comment	Textmining
Clostridioides difficile	-	gene pdeH	-
Clostridioides difficile 360	-	gene pdeH	-

Synonyms

Synonyms	Comment	Organism
c-di-GMP phosphodiesterase	-	Clostridioides difficile
PdeH	-	Clostridioides difficile

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Release 2023.1 (January 2023)