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Literature summary for 3.1.4.52 extracted from

  • An, S.; Wu, J.; Zhang, L.H.
    Modulation of Pseudomonas aeruginosa biofilm dispersal by a cyclic-di-GMP phosphodiesterase with a putative hypoxia-sensing domain (2010), Appl. Environ. Microbiol., 76, 8160-8173.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.52

Cloned(Commentary)

Cloned (Comment) Organism
gene rbdA, expression as GST-tagged enzyme in Escherichia coli Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
additional information deletion of the PAS domain or substitution of the key residues implicated in sensing low-oxygen stress abrogates the functionality of RbdA Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cyclic di-3',5'-guanylate + H2O Pseudomonas aeruginosa
-
 5'-phosphoguanylyl(3'-5')guanosine
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
 gene rbdA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged enzyme from Escherichia coli by glutathione affinity chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclic di-3',5'-guanylate + H2O
-
 Pseudomonas aeruginosa 5'-phosphoguanylyl(3'-5')guanosine
-
 ?

Synonyms

Synonyms Comment Organism
RbdA
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Pseudomonas aeruginosa

General Information

General Information Comment Organism
malfunction mutation of rbdA, which encodes a fusion protein consisting of PAS-PAC-GGDEF-EAL multidomains, results in decreased biofilm dispersal Pseudomonas aeruginosa
additional information mutation of PA0861 encoding a multidomain regulatory protein implicated in cyclic di-GMP metabolism resulted in a hyperbiofilm phenotype Pseudomonas aeruginosa
physiological function RbdA is involved in positive regulation of bacterial motility and production of rhamnolipids, which are associated with biofilm dispersal, and in negative regulation of production of exopolysaccharides, which are required for biofilm formation. The cyclic di-GMP-degrading regulatory protein RbdA promotes biofilm dispersal through its two-pronged effects on biofilm development, i.e., downregulating biofilm formation and upregulating production of the factors associated with biofilm dispersal.. RbdA contains a highly conserved GGDEF domain and EAL domain, which are involved in the synthesis and degradation of cyclic di-GMP, respectively. The full-length RbdA protein only displays phosphodiesterase activity, causing cyclic di-GMP degradation Pseudomonas aeruginosa