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Literature summary for 3.1.4.52 extracted from

  • Barends, T.R.; Hartmann, E.; Griese, J.J.; Beitlich, T.; Kirienko, N.V.; Ryjenkov, D.A.; Reinstein, J.; Shoeman, R.L.; Gomelsky, M.; Schlichting, I.
    Structure and mechanism of a bacterial light-regulated cyclic nucleotide phosphodiesterase (2009), Nature, 459, 1015-1018.
    View publication on PubMed
Search for more literature for 3.1.4.52

Cloned(Commentary)

Cloned (Comment) Organism
-
 Klebsiella pneumoniae

Crystallization (Commentary)

Crystallization (Comment) Organism
BlrP1 is crystallized at 4°C in the dark using polyethyleneglycol in the presence of cyclic-di-GMP and Ca2+ Klebsiella pneumoniae

Protein Variants

Protein Variants Comment Organism
L128C/G353C the mutant shows higher catalytic efficiency at pH 7.5 and 20°C compared to the wild type enzyme under light and dark conditions Klebsiella pneumoniae
R93S the mutant shows higher catalytic efficiency at pH 7.5 and 20°C compared to the wild type enzyme under light and dark conditions Klebsiella pneumoniae
S309C/S312C inactive at pH 7.5 and 20°C under dark and light conditions Klebsiella pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ complete inhibition at 10 mM Klebsiella pneumoniae
Cu2+ complete inhibition at 10 mM Klebsiella pneumoniae
Zn2+ complete inhibition at 10 mM Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclic di-3',5'-guanylate + H2O
-
 Klebsiella pneumoniae 5'-phosphoguanylyl(3'-5')guanosine
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Klebsiella pneumoniae

Synonyms

Synonyms Comment Organism
BlrP1 also known as KPN 01598, BlrP1 consists of a BLUF sensor domain and a phosphodiesterase EAL output domain which hydrolyses cyclic dimeric GMP, so BlrP1 possesses c-di-GMP-phosphodiesterase activity Klebsiella pneumoniae
cyclic nucleotide phosphodiesterase
-
 Klebsiella pneumoniae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.13
-
 cyclic di-3',5'-guanylate wild type enzyme, at pH 7.5, 20°C, under dark conditions Klebsiella pneumoniae
0.27
-
 cyclic di-3',5'-guanylate mutant enzyme R93S, at pH 7.5, 20°C, under dark conditions Klebsiella pneumoniae
0.54
-
 cyclic di-3',5'-guanylate wild type enzyme, at pH 7.5, 20°C, under light conditions Klebsiella pneumoniae
0.64
-
 cyclic di-3',5'-guanylate wild type enzyme, in the presence of 10 mM Mn2+, at pH 7.5, 20°C, under dark conditions Klebsiella pneumoniae
1.17
-
 cyclic di-3',5'-guanylate wild type enzyme, in the presence of 10 mM Mn2+, at pH 7.5, 20°C, under light conditions Klebsiella pneumoniae
1.42
-
 cyclic di-3',5'-guanylate mutant enzyme R93S, at pH 7.5, 20°C, under light conditions Klebsiella pneumoniae
1.84
-
 cyclic di-3',5'-guanylate wild type enzyme, at pH 8.0, 20°C, under dark conditions Klebsiella pneumoniae
2.06
-
 cyclic di-3',5'-guanylate mutant enzyme L128C/G353C, at pH 7.5, 20°C, under dark conditions Klebsiella pneumoniae
2.54
-
 cyclic di-3',5'-guanylate wild type enzyme, at pH 8.0, 20°C, under light conditions Klebsiella pneumoniae
3.48
-
 cyclic di-3',5'-guanylate mutant enzyme L128C/G353C, at pH 7.5, 20°C, under light conditions Klebsiella pneumoniae
6.17
-
 cyclic di-3',5'-guanylate wild type enzyme, at pH 9.3, 20°C, under light conditions Klebsiella pneumoniae
6.7
-
 cyclic di-3',5'-guanylate wild type enzyme, at pH 9.3, 20°C, under dark conditions Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
Calmodulin
-
 Klebsiella pneumoniae