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Literature summary for 3.1.4.52 extracted from

  • Rao, F.; Qi, Y.; Chong, H.S.; Kotaka, M.; Li, B.; Li, J.; Lescar, J.; Tang, K.; Liang, Z.X.
    The functional role of a conserved loop in EAL domain-based cyclic di-GMP-specific phosphodiesterase (2009), J. Bacteriol., 191, 4722-4731.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.52

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
D296A the RocR mutant shows decreased kcat and increased Km compared to the wild type RocR enzyme Pseudomonas aeruginosa
D56N the RocR mutant exhibits a significantly smaller Km value than that of the wild type enzyme Pseudomonas aeruginosa
E268A the RocR mutation reduces the enzyme activity to below the measurable level Pseudomonas aeruginosa
E268Q the RocR mutation causes a 446fold reduction in kcat Pseudomonas aeruginosa
E464A the PA2567 mutant shows increased kcat and Km compared to the wild type enzyme Pseudomonas aeruginosa
F297A the RocR mutant shows decreased kcat and Km compared to the wild type RocR enzyme Pseudomonas aeruginosa
F498A the PA2567 mutation reduces the enzyme activity to below the measurable level Pseudomonas aeruginosa
S302A the RocR mutant shows decreased kcat and increased Km compared to the wild type RocR enzyme Pseudomonas aeruginosa
S493A the PA2567 mutation reduces the enzyme activity to below the measurable level Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0002
-
 cyclic di-3',5'-guanylate mutant RocR enzyme D56N, in 100 mM Tris buffer (pH 8.0) (23°C), 20 mM KCl, and 25 mM MgCl2 Pseudomonas aeruginosa
0.0003
-
 cyclic di-3',5'-guanylate mutant RocR enzyme E268Q, in 100 mM Tris buffer (pH 8.0) (23°C), 20 mM KCl, and 25 mM MgCl2 Pseudomonas aeruginosa
0.0007
-
 cyclic di-3',5'-guanylate mutant RocR enzyme F297A, in 100 mM Tris buffer (pH 8.0) (23°C), 20 mM KCl, and 25 mM MgCl2 Pseudomonas aeruginosa
0.0032
-
 cyclic di-3',5'-guanylate wild type RocR enzyme, in 100 mM Tris buffer (pH 8.0) (23°C), 20 mM KCl, and 25 mM MgCl2 Pseudomonas aeruginosa
0.0052
-
 cyclic di-3',5'-guanylate wild type PA2567 enzyme, in 100 mM Tris buffer (pH 8.0) (23°C), 50 mM KCl, and 10 mM MgCl2 Pseudomonas aeruginosa
0.0055
-
 cyclic di-3',5'-guanylate mutant RocR enzyme S302A, in 100 mM Tris buffer (pH 8.0) (23°C), 20 mM KCl, and 25 mM MgCl2 Pseudomonas aeruginosa
0.0086
-
 cyclic di-3',5'-guanylate mutant RocR enzyme D296A, in 100 mM Tris buffer (pH 8.0) (23°C), 20 mM KCl, and 25 mM MgCl2 Pseudomonas aeruginosa
0.0094
-
 cyclic di-3',5'-guanylate mutant RocR enzyme D56N/D296A, in 100 mM Tris buffer (pH 8.0) (23°C), 20 mM KCl, and 25 mM MgCl2 Pseudomonas aeruginosa
0.013
-
 cyclic di-3',5'-guanylate mutant PA2567 enzyme E464A, in 100 mM Tris buffer (pH 8.0) (23°C), 50 mM KCl, and 10 mM MgCl2 Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclic di-3',5'-guanylate + H2O
-
 Pseudomonas aeruginosa 5'-phosphoguanylyl(3'-5')guanosine
-
 ?

Synonyms

Synonyms Comment Organism
c-di-GMP-specific phosphodiesterase
-
 Pseudomonas aeruginosa
cyclic di-GMP-specific phosphodiesterase
-
 Pseudomonas aeruginosa
PA2567
-
 Pseudomonas aeruginosa
PDE
-
 Pseudomonas aeruginosa
RocR
-
 Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0015
-
 cyclic di-3',5'-guanylate mutant RocR enzyme E268Q, in 100 mM Tris buffer (pH 8.0) (23°C), 20 mM KCl, and 25 mM MgCl2 Pseudomonas aeruginosa
0.012
-
 cyclic di-3',5'-guanylate mutant RocR enzyme D56N/D296A, in 100 mM Tris buffer (pH 8.0) (23°C), 20 mM KCl, and 25 mM MgCl2 Pseudomonas aeruginosa
0.02
-
 cyclic di-3',5'-guanylate mutant RocR enzyme F297A, in 100 mM Tris buffer (pH 8.0) (23°C), 20 mM KCl, and 25 mM MgCl2 Pseudomonas aeruginosa
0.021
-
 cyclic di-3',5'-guanylate mutant RocR enzyme D296A, in 100 mM Tris buffer (pH 8.0) (23°C), 20 mM KCl, and 25 mM MgCl2 Pseudomonas aeruginosa
0.13
-
 cyclic di-3',5'-guanylate mutant RocR enzyme D56N, in 100 mM Tris buffer (pH 8.0) (23°C), 20 mM KCl, and 25 mM MgCl2 Pseudomonas aeruginosa
0.14
-
 cyclic di-3',5'-guanylate mutant RocR enzyme S302A, in 100 mM Tris buffer (pH 8.0) (23°C), 20 mM KCl, and 25 mM MgCl2 Pseudomonas aeruginosa
0.39
-
 cyclic di-3',5'-guanylate wild type PA2567 enzyme, in 100 mM Tris buffer (pH 8.0) (23°C), 50 mM KCl, and 10 mM MgCl2 Pseudomonas aeruginosa
0.67
-
 cyclic di-3',5'-guanylate wild type RocR enzyme, in 100 mM Tris buffer (pH 8.0) (23°C), 20 mM KCl, and 25 mM MgCl2 Pseudomonas aeruginosa
1.1
-
 cyclic di-3',5'-guanylate mutant PA2567 enzyme E464A, in 100 mM Tris buffer (pH 8.0) (23°C), 50 mM KCl, and 10 mM MgCl2 Pseudomonas aeruginosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1
-
 cyclic di-3',5'-guanylate mutant RocR enzyme D56N/D296A, in 100 mM Tris buffer (pH 8.0) (23°C), 20 mM KCl, and 25 mM MgCl2 Pseudomonas aeruginosa
2.7
-
 cyclic di-3',5'-guanylate mutant RocR enzyme D296A, in 100 mM Tris buffer (pH 8.0) (23°C), 20 mM KCl, and 25 mM MgCl2 Pseudomonas aeruginosa
4.8
-
 cyclic di-3',5'-guanylate mutant RocR enzyme E268Q, in 100 mM Tris buffer (pH 8.0) (23°C), 20 mM KCl, and 25 mM MgCl2 Pseudomonas aeruginosa
25
-
 cyclic di-3',5'-guanylate mutant RocR enzyme S302A, in 100 mM Tris buffer (pH 8.0) (23°C), 20 mM KCl, and 25 mM MgCl2 Pseudomonas aeruginosa
28
-
 cyclic di-3',5'-guanylate mutant RocR enzyme F297A, in 100 mM Tris buffer (pH 8.0) (23°C), 20 mM KCl, and 25 mM MgCl2 Pseudomonas aeruginosa
75
-
 cyclic di-3',5'-guanylate wild type PA2567 enzyme, in 100 mM Tris buffer (pH 8.0) (23°C), 50 mM KCl, and 10 mM MgCl2 Pseudomonas aeruginosa
84
-
 cyclic di-3',5'-guanylate mutant PA2567 enzyme E464A, in 100 mM Tris buffer (pH 8.0) (23°C), 50 mM KCl, and 10 mM MgCl2 Pseudomonas aeruginosa
210
-
 cyclic di-3',5'-guanylate wild type RocR enzyme, in 100 mM Tris buffer (pH 8.0) (23°C), 20 mM KCl, and 25 mM MgCl2 Pseudomonas aeruginosa
650
-
 cyclic di-3',5'-guanylate mutant RocR enzyme D56N, in 100 mM Tris buffer (pH 8.0) (23°C), 20 mM KCl, and 25 mM MgCl2 Pseudomonas aeruginosa