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Literature summary for 3.1.4.52 extracted from

  • Kazmierczak, B.I.; Lebron, M.B.; Murray, T.S.
    Analysis of FimX, a phosphodiesterase that governs twitching motility in Pseudomonas aeruginosa (2006), Mol. Microbiol., 60, 1026-1043.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
GTP binds to the GGDEF domain which in turn activates the EAL domain Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
D347A mutant is indistinguishable from enzyme null mutant. Loss of twitching activity and PO4 phage sensitivity, mutant fails to assemble surface pili Pseudomonas aeruginosa
E475A mutant is indistinguishable from enzyme null mutant. Loss of twitching activity and PO4 phage sensitivity, mutant fails to assemble surface pili Pseudomonas aeruginosa
G346A mutant is indistinguishable from enzyme null mutant. Loss of twitching activity and PO4 phage sensitivity, mutant fails to assemble surface pili Pseudomonas aeruginosa
L477A mutant is indistinguishable from enzyme null mutant. Loss of twitching activity and PO4 phage sensitivity, mutant fails to assemble surface pili Pseudomonas aeruginosa
additional information deletion of either the GGDEF or the EAL domain results in a phenotype indistinguishable from enzyme deletion mutant. Deletion of the amino-terminal REC domain containing a putative polar localization signal results in a protein that still supports intermediate levels of pilus assembly and function, but is no longer localized to the bacterial pole. Enzyme deletion mutant is as virulent as wild-type in a murine model of acute pneumonia Pseudomonas aeruginosa
V476A mutant is indistinguishable from enzyme null mutant. Loss of twitching activity and PO4 phage sensitivity, mutant fails to assemble surface pili Pseudomonas aeruginosa

Localization

Localization Comment Organism GeneOntology No. Textmining
cell pole restriction of protein to a single pole requires intact GGDEF and EAL domains Pseudomonas aeruginosa 60187
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Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
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protein FimX with both GGDEF and EAL domains. FimX is involved in assembly of type IV pili
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclic di-3',5'-guanylate + H2O enzyme has no diguanylate cyclase activity Pseudomonas aeruginosa 5'-phosphoguanylyl(3'-5')guanosine
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Synonyms

Synonyms Comment Organism
FimX
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Pseudomonas aeruginosa