D347A |
mutant is indistinguishable from enzyme null mutant. Loss of twitching activity and PO4 phage sensitivity, mutant fails to assemble surface pili |
Pseudomonas aeruginosa |
E475A |
mutant is indistinguishable from enzyme null mutant. Loss of twitching activity and PO4 phage sensitivity, mutant fails to assemble surface pili |
Pseudomonas aeruginosa |
G346A |
mutant is indistinguishable from enzyme null mutant. Loss of twitching activity and PO4 phage sensitivity, mutant fails to assemble surface pili |
Pseudomonas aeruginosa |
L477A |
mutant is indistinguishable from enzyme null mutant. Loss of twitching activity and PO4 phage sensitivity, mutant fails to assemble surface pili |
Pseudomonas aeruginosa |
additional information |
deletion of either the GGDEF or the EAL domain results in a phenotype indistinguishable from enzyme deletion mutant. Deletion of the amino-terminal REC domain containing a putative polar localization signal results in a protein that still supports intermediate levels of pilus assembly and function, but is no longer localized to the bacterial pole. Enzyme deletion mutant is as virulent as wild-type in a murine model of acute pneumonia |
Pseudomonas aeruginosa |
V476A |
mutant is indistinguishable from enzyme null mutant. Loss of twitching activity and PO4 phage sensitivity, mutant fails to assemble surface pili |
Pseudomonas aeruginosa |