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Literature summary for 3.1.4.52 extracted from

  • Schmidt, A.J.; Ryjenkov, D.A.; Gomelsky, M.
    The ubiquitous protein domain EAL is a cyclic diguanylate-specific phosphodiesterase: enzymatically active and inactive EAL domains (2005), J. Bacteriol., 187, 4774-4781.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.52

Cloned(Commentary)

Cloned (Comment) Organism
overexpression with His-tag Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information both full-length enzyme and the isolated EAL domain hydrolyze cyclic diguanylate. Hydrolysis of 5’-phosphoguanylyl(3’-5’)guanosine to guanosine monophosphate by isolated domain EAL is very poor Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ strong Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 cyclic di-3',5'-guanylate pH 9.4 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 10% of the activity with Mg2+, Mn2+ in presence of Co2+ Escherichia coli
Mg2+ or Mn2+, absolutely required, with Mn2+ being slightly more efficient Escherichia coli
Mn2+ or Mg2+, absolutely required, with Mn2+ being slightly more efficient Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 isoform YahA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged protein Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclic di-3',5'-guanylate + H2O specific substrate Escherichia coli 5'-phosphoguanylyl(3'-5')guanosine
-
 ?

Synonyms

Synonyms Comment Organism
YahA
-
 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.4
-
-
 Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
10
-
 slight decrease in activity when raising pH value from 9.4 to 10 Escherichia coli