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Literature summary for 3.1.4.46 extracted from

  • Pedroso, M.M.; Ely, F.; Carpenter, M.C.; Mitic, N.; Gahan, L.R.; Ollis, D.L.; Wilcox, D.E.; Schenk, G.
    Mechanistic insight from calorimetric measurements of the assembly of the binuclear metal active site of glycerophosphodiesterase (GpdQ) from Enterobacter aerogenes (2017), Biochemistry, 56, 3328-3336 .
    View publication on PubMed
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Activating Compound

Activating Compound Comment Organism Structure
phosphate the presence of phosphate increases the affinity of the alpha site for Mn(II) by about 30fold and its affinity for Co(II) more than 70fold Klebsiella aerogenes

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Klebsiella aerogenes

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ quantification of binding affinity. Metal ions bind to the six-coordinate alpha site of the protein in an entropically driven process with loss of a proton, while binding at the beta site is not detected. Phosphate enhances the metal affinity of the alpha site by increasing the binding entropy and the metal affinity of the beta site by enthalpic (Co) or entropic (Mn) contributions, but no additional loss of protons Klebsiella aerogenes
Mn2+ quantification of binding affinity. Metal ions bind to the six-coordinate alpha site of the protein in an entropically driven process with loss of a proton, while binding at the beta site is not detected. Phosphate enhances the metal affinity of the alpha site by increasing the binding entropy and the metal affinity of the beta site by enthalpic (Co) or entropic (Mn) contributions, but no additional loss of protons Klebsiella aerogenes

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the metal-bound hydroxide nucleophile is formed in the resting inactive mononuclear form, which becomes catalytically competent upon binding the second metal ion Klebsiella aerogenes ?
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