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Literature summary for 3.1.4.46 extracted from
- Crystal structure of glycerophosphodiester phosphodiesterase from Agrobacterium tumefaciens by SAD with a large asymmetric unit (2006), Proteins Struct. Funct. Bioinform., 65, 514-518.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed as His-tag fusion protein in Escherichia coli TOP10 cells | Agrobacterium tumefaciens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| sitting drop vapor diffusion method | Agrobacterium tumefaciens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27300 | - |
6 * 27300, crystal structure analysis | Agrobacterium tumefaciens |
| 27300 | - |
x * 27300, gel filtration experiments with an estimated molecular weight of approximately 160000 Da indicates a higher number of monomers than found in crystals | Agrobacterium tumefaciens |
| 160000 | - |
a molecular weight of approximately 160000 Da estimated by gel filtration | Agrobacterium tumefaciens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycerophosphodiester + H2O | Agrobacterium tumefaciens | - |
glycerol 3-phosphate + alcohol | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agrobacterium tumefaciens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein using His-tag | Agrobacterium tumefaciens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycerophosphodiester + H2O | - |
Agrobacterium tumefaciens | glycerol 3-phosphate + alcohol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 27300, gel filtration experiments with an estimated molecular weight of approximately 160000 Da indicates a higher number of monomers than found in crystals | Agrobacterium tumefaciens |
| hexamer | 6 * 27300, crystal structure analysis | Agrobacterium tumefaciens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GDPD | - |
Agrobacterium tumefaciens |
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Release 2023.1 (January 2023)
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