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Literature summary for 3.1.4.46 extracted from
- Escherichia coli cytosolic glycerophosphodiester phosphodiesterase (UgpQ) requires Mg2+, Co2+, or Mn2+ for its enzyme activity (2008), J. Bacteriol., 190, 1219-1223.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) | Escherichia coli K-12 |
| protein in Escherichia coli BL21(DE3) | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.22 | - |
glycerophosphoethanolamine | - |
Escherichia coli K-12 |  |
| 0.22 | - |
glycerophosphoethanolamine | 5 mM Mg2+, pH 7.4, 35°C | Escherichia coli | |
| 0.39 | - |
glycerophosphoinositol | - |
Escherichia coli K-12 | |
| 0.39 | - |
glycerophosphoinositol | 5 mM Mg2+, pH 7.4, 35°C | Escherichia coli | |
| 0.62 | - |
glycerophosphoglycerol | - |
Escherichia coli K-12 | |
| 0.62 | - |
glycerophosphoglycerol | 5 mM Mg2+, pH 7.4, 35°C | Escherichia coli | |
| 0.66 | - |
glycerophosphoserine | - |
Escherichia coli K-12 | |
| 0.66 | - |
glycerophosphoserine | 5 mM Mg2+, pH 7.4, 35°C | Escherichia coli | |
| 2 | - |
glycerophosphocholine | - |
Escherichia coli K-12 | |
| 2 | - |
glycerophosphocholine | 5 mM Mg2+, pH 7.4, 35°C | Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Escherichia coli K-12 | 5829 | - |
| cytosol | - |
Escherichia coli | 5829 | - |
| periplasm | - |
Escherichia coli | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required | Escherichia coli | |
| Co2+ | can replace Mg2+ | Escherichia coli | |
| Co2+ | requires Mg2+, Mn2+, or Co2+ | Escherichia coli K-12 | |
| Mg2+ | required | Escherichia coli | |
| Mg2+ | requires Mg2+, Mn2+, or Co2+ | Escherichia coli K-12 | |
| Mn2+ | can replace Mg2+ | Escherichia coli | |
| Mn2+ | requires Mg2+, Mn2+, or Co2+ | Escherichia coli K-12 | |
| additional information | Ca2+, Cu2+, Ni2+, Cd2+, Zn2+, and Fe2+ are ineffective | Escherichia coli | |
| additional information | Mg2+, Mn2+, Co2+, Cu2+, Ni2+, Cd2+, Zn2+, and Fe2+ are ineffective | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
x * 27000, SDS-PAGE | Escherichia coli K-12 |
| 27000 | - |
x * 27000, SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycerophosphocholine + H2O | Escherichia coli | - |
sn-glycerol 3-phosphate + choline | - |
? | |
| glycerophosphodiester + H2O | Escherichia coli | - |
sn-glycerol 3-phosphate + alcohol | sn-glycerol-3-phosphate supply is essential for the de novo synthesis of glycerophospholipids | ? | |
| glycerophosphodiester + H2O | Escherichia coli | UgpQ accumulates under conditions of phosphate starvation suggesting a role in the utilization of phosphate from glycerophosphodiesters | sn-glycerol 3-phosphate + alcohol | sn-glycerol-3-phosphate supply is essential for the de novo synthesis of glycerophospholipids | ? | |
| glycerophosphoethanolamine + H2O | Escherichia coli | - |
sn-glycerol 3-phosphate + ethanolamine | - |
? | |
| glycerophosphoglycerol + H2O | Escherichia coli | - |
sn-glycerol 3-phosphate + glycerol | - |
? | |
| glycerophosphoinositol + H2O | Escherichia coli | - |
sn-glycerol 3-phosphate + inositol | - |
? | |
| glycerophosphoserine + H2O | Escherichia coli | - |
sn-glycerol 3-phosphate + serine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P09394 | - |
- |
| Escherichia coli | P10908 | - |
- |
| Escherichia coli K-12 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ion exchange chromatography, hydroxyapatite chromatography, gel filtration | Escherichia coli K-12 |
| recombinant protein | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycerophosphocholine + H2O | - |
Escherichia coli K-12 | sn-glycerol 3-phosphate + choline | - |
? | |
| glycerophosphocholine + H2O | - |
Escherichia coli | sn-glycerol 3-phosphate + choline | - |
? | |
| glycerophosphodiester + H2O | - |
Escherichia coli | sn-glycerol 3-phosphate + alcohol | sn-glycerol-3-phosphate supply is essential for the de novo synthesis of glycerophospholipids | ? | |
| glycerophosphodiester + H2O | UgpQ accumulates under conditions of phosphate starvation suggesting a role in the utilization of phosphate from glycerophosphodiesters | Escherichia coli | sn-glycerol 3-phosphate + alcohol | sn-glycerol-3-phosphate supply is essential for the de novo synthesis of glycerophospholipids | ? | |
| glycerophosphoethanolamine + H2O | - |
Escherichia coli K-12 | sn-glycerol 3-phosphate + ethanolamine | - |
? | |
| glycerophosphoethanolamine + H2O | - |
Escherichia coli | sn-glycerol 3-phosphate + ethanolamine | - |
? | |
| glycerophosphoglycerol + H2O | - |
Escherichia coli K-12 | sn-glycerol 3-phosphate + glycerol | - |
? | |
| glycerophosphoglycerol + H2O | - |
Escherichia coli | sn-glycerol 3-phosphate + glycerol | - |
? | |
| glycerophosphoinositol + H2O | - |
Escherichia coli K-12 | ? | - |
? | |
| glycerophosphoinositol + H2O | - |
Escherichia coli | sn-glycerol 3-phosphate + inositol | - |
? | |
| glycerophosphoserine + H2O | - |
Escherichia coli K-12 | ? | - |
? | |
| glycerophosphoserine + H2O | - |
Escherichia coli | sn-glycerol 3-phosphate + serine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 27000, SDS-PAGE | Escherichia coli K-12 |
| ? | x * 27000, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GlpQ | periplasmic enzyme, requires Ca2+ | Escherichia coli |
| glycerophosphodiester phosphodiesterase | - |
Escherichia coli K-12 |
| UgpQ | cytosolic enzyme, requires Mg2+, Mn2+, or Co2+ | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.1 | - |
glycerophosphoethanolamine | - |
Escherichia coli K-12 | |
| 1.1 | - |
glycerophosphoethanolamine | 5 mM Mg2+, pH 7.4, 35°C | Escherichia coli | |
| 1.8 | - |
glycerophosphoinositol | - |
Escherichia coli K-12 | |
| 1.8 | - |
glycerophosphoinositol | 5 mM Mg2+, pH 7.4, 35°C | Escherichia coli | |
| 2.8 | - |
glycerophosphoserine | - |
Escherichia coli K-12 | |
| 2.8 | - |
glycerophosphoserine | UgpQ, 5 mM Mg2+, pH 7.4, 35°C | Escherichia coli | |
| 3.2 | - |
glycerophosphocholine | - |
Escherichia coli K-12 | |
| 3.2 | - |
glycerophosphocholine | 5 mM Mg2+, pH 7.4, 35°C | Escherichia coli | |
| 3.3 | - |
glycerophosphoglycerol | - |
Escherichia coli K-12 | |
| 3.3 | - |
glycerophosphoglycerol | 5 mM Mg2+, pH 7.4, 35°C | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Escherichia coli K-12 |
| 7.5 | - |
- |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9.5 | - |
Escherichia coli K-12 |
| 6 | 9 | - |
Escherichia coli |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.6 | - |
glycerophosphocholine | - |
Escherichia coli K-12 | |
| 4.2 | - |
glycerophosphoserine | - |
Escherichia coli K-12 | |
| 4.7 | - |
glycerophosphoinositol | - |
Escherichia coli K-12 | |
| 4.8 | - |
glycerophosphoethanolamine | - |
Escherichia coli K-12 | |
| 5.4 | - |
glycerophosphoglycerol | - |
Escherichia coli K-12 | |
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