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Literature summary for 3.1.4.46 extracted from

  • Jackson, C.J.; Carr, P.D.; Kim, H.K.; Liu, J.W.; Ollis, D.L.
    The purification, crystallization and preliminary diffraction of a glycerophosphodiesterase from Enterobacter aerogenes (2006), Acta Crystallogr. Sect. F, 62, 659-661.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.46

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha Klebsiella aerogenes

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, best crystallization results when apo-GpdQ is used, crystals with bound Zn2+ and Co2+ Klebsiella aerogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 2 * 31000, SDS-PAGE, crystal structure analysis Klebsiella aerogenes
3083993
-
 2 * 3083993, calculated from the deduced amino acid sequence, crystal structure analysis Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycerophosphocholine + H2O Klebsiella aerogenes maintenance of the sn-glycerol-3-phosphate concentration which is essential to enable phospholipid synthesis sn-glycerol 3-phosphate + choline
-
 ?
glycerophosphodiester + H2O Klebsiella aerogenes maintenance of the sn-glycerol-3-phosphate concentration which is essential to enable phospholipid synthesis sn-glycerol 3-phosphate + alcohol
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes Q6XBH1
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycerophosphocholine + H2O maintenance of the sn-glycerol-3-phosphate concentration which is essential to enable phospholipid synthesis Klebsiella aerogenes sn-glycerol 3-phosphate + choline
-
 ?
glycerophosphodiester + H2O maintenance of the sn-glycerol-3-phosphate concentration which is essential to enable phospholipid synthesis Klebsiella aerogenes sn-glycerol 3-phosphate + alcohol
-
 ?

Subunits

Subunits Comment Organism
? data also indicate the possibility of a tetrameric organization or other number of monomers Klebsiella aerogenes
dimer 2 * 3083993, calculated from the deduced amino acid sequence, crystal structure analysis Klebsiella aerogenes
dimer 2 * 31000, SDS-PAGE, crystal structure analysis Klebsiella aerogenes

Synonyms

Synonyms Comment Organism
GpdQ
-
 Klebsiella aerogenes
metallo-glycerophosphodiesterase
-
 Klebsiella aerogenes