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Literature summary for 3.1.4.40 extracted from

  • Van Dijk, W.; Maier, H.; Van den Eijnden, D.H.
    Properties and subcellular localization of CMP-N-acetylneuraminic acid hydrolase of calf kidney (1976), Biochim. Biophys. Acta, 444, 816-834.
    View publication on PubMed
Search for more literature for 3.1.4.40

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 increase of enzyme activity Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
 Bos taurus
CMP 40% inhibition at low CMP-N-acylneuraminate substrate concentrations Bos taurus
cytidine 40% inhibition at low CMP-N-acylneuraminate substrate concentrations Bos taurus
EDTA total loss of activity at 1 mM Bos taurus
Mn2+ slight inhibition at lower concentrations Bos taurus
UDP-N-acetylglucosamine
-
 Bos taurus
UDPgalactose
-
 Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.47
-
 CMP-N-acylneuraminate
-
 Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Bos taurus
-
-
plasma membrane activity in subfractions Bos taurus 5886
-
plasma membrane of kidney Bos taurus 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stimulation Bos taurus
divalent cations required, monovalent metal ions without or little stimulatory effect Bos taurus
Mg2+ stimulation Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CMP-N-acylneuraminate + H2O Bos taurus
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 calf
-

Source Tissue

Source Tissue Comment Organism Textmining
kidney cortex Bos taurus
-
kidney medulla Bos taurus
-
kidney papilla Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bos taurus

Storage Stability

Storage Stability Organism
-20°C, for at least 5 months, no loss of activity Bos taurus
0-4°C, for at several days, no loss of activity Bos taurus
repeated freezing and thawing, no influence of enzyme activity Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CMP-N-acylneuraminate + H2O
-
 Bos taurus CMP + N-acylneuraminate
-
 ?
CMP-N-acylneuraminate + H2O
-
 Bos taurus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8 9
-
 Bos taurus