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Literature summary for 3.1.4.4 extracted from

  • Khatoon, H.; Mansfeld, J.; Schierhorn, A.; Ulbrich-Hofmann, R.
    Purification, sequencing and characterization of phospholipase D from Indian mustard seeds (2015), Phytochemistry, 117, 65-75 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
phosphatidylinositol 4,5-bisphosphate strong activating effect at 2-10 mM CaCl2 Brassica juncea var. juncea

Inhibitors

Inhibitors Comment Organism Structure
Na3VO4
-
Brassica juncea var. juncea
NaAlF4
-
Brassica juncea var. juncea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.84
-
phosphatidylcholine pH 5.5, 37°C Brassica juncea var. juncea

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ maximum activity above 60 mM Brassica juncea var. juncea

Organism

Organism UniProt Comment Textmining
Brassica juncea var. juncea A0A0G4AKV1
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
43.6
-
substrate phosphatidylglycerol, pH 5.5, 37°C Brassica juncea var. juncea
117.8
-
substrate phosphatidylethanolamine, pH 5.5, 37°C Brassica juncea var. juncea
253.8
-
substrate phosphatidylcholine, pH 5.5, 37°C Brassica juncea var. juncea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information phosphatidylcholines with short-chain fatty acids are better substrates than phosphatidylcholines with long fatty acid chains. Lysophosphatidylcholine is not accepted as substrate Brassica juncea var. juncea ?
-
?
phosphatidylcholine + H2O
-
Brassica juncea var. juncea choline + phosphatidate
-
?
phosphatidylethanolamine + H2O
-
Brassica juncea var. juncea ethanolamine + phosphatidate
-
?
phosphatidylglycerol + H2O
-
Brassica juncea var. juncea glycerol + phosphatidate
-
?

Subunits

Subunits Comment Organism
? x * 92000, SDS-PAGE and calculated from sequence Brassica juncea var. juncea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
inactivation above Brassica juncea var. juncea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6
-
Brassica juncea var. juncea

pI Value

Organism Comment pI Value Maximum pI Value
Brassica juncea var. juncea calculated from sequence
-
5.4

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0078
-
pH 5.5, 37°C Brassica juncea var. juncea NaAlF4
0.033
-
pH 5.5, 37°C Brassica juncea var. juncea Na3VO4