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Literature summary for 3.1.4.4 extracted from

  • Zhou, W.B.; Gong, J.S.; Hou, H.J.; Li, H.; Lu, Z.M.; Xu, H.Y.; Xu, Z.H.; Shi, J.S.
    Mining of a phospholipase D and its application in enzymatic preparation of phosphatidylserine (2018), Bioengineered, 9, 80-89 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces mobaraensis

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 5 mM, 25% inhibition Streptomyces mobaraensis
Ag+ 5 mM, 77% inhibition Streptomyces mobaraensis
dithiothreitol 5 mM, 20% inhibition Streptomyces mobaraensis
EDTA 5 mM, 94% inhibition Streptomyces mobaraensis
Fe3+ 5 mM, 44% inhibition Streptomyces mobaraensis
PMSF 5 mM, 60% inhibition Streptomyces mobaraensis
SDS 5 mM, 50% inhibition Streptomyces mobaraensis

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 5 mM, 118% of initial activity Streptomyces mobaraensis
Ca2+ 5 mM, 134% of initial activity Streptomyces mobaraensis
Co2+ 5 mM, 118% of initial activity Streptomyces mobaraensis

Organism

Organism UniProt Comment Textmining
Streptomyces mobaraensis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme displays transphosphatidylation activity, and phosphatidylserine can be synthesized with L-serine and soybean lecithin as substrates Streptomyces mobaraensis ?
-
 ?
phosphatidylcholine + H2O substrate soybean lecithin Streptomyces mobaraensis choline + a phosphatidate
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, calculated from sequence and SDS-PAGE Streptomyces mobaraensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Streptomyces mobaraensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40
-
 50% of maximum activity Streptomyces mobaraensis
80
-
 60% of maximum activity Streptomyces mobaraensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 60 min, 79% residual activity Streptomyces mobaraensis
55
-
 stable up to Streptomyces mobaraensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Streptomyces mobaraensis

pH Range

pH Minimum pH Maximum Comment Organism
4 10 low activity above or below Streptomyces mobaraensis