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Literature summary for 3.1.4.4 extracted from
- Biochemical characterization of a Pseudomonas aeruginosa phospholipase D (2015), Biochemistry, 54, 1208-1218 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | inhibitory at 5 mM and 10 mM, substrate dioleoylphosphatidylethanolamine, stimulation at 2.5 mM, substrate dioleoylphosphatidylcholine | Pseudomonas aeruginosa |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | inhibitory at 5 mM and 10 mM, substrate dioleoylphosphatidylethanolamine. Presence of Ca2+ is required for substrate dioleoylphosphatidylcholine | Pseudomonas aeruginosa | |
| Mg2+ | 10 mM, 1.6fold increase in activity, substrate dioleoylphosphatidylethanolamine. Presence of Mg2+ is required for substrate dioleoylphosphatidylcholine | Pseudomonas aeruginosa | |
| additional information | stimulation by divalent cation depends on the substrate | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1345 | - |
substrate oleoylphosphatidylethanolamine, pH 7.5, 37°C | Pseudomonas aeruginosa |
| 2871 | - |
substrate dioleoylphosphatidylethanolamine, pH 7.5, 37°C | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-oleoyl-2-stearoylphosphatidylethanolamine + H2O | - |
Pseudomonas aeruginosa | ethanolamine + 1-oleoyl-2-stearoyl-sn-glycero-3-phosphatidic acid | - |
? | |
| dioleoylphosphatidylcholine + H2O | - |
Pseudomonas aeruginosa | choline + 1,2-dioleoyl-sn-glycero-3-phosphatidic acid | - |
? | |
| dioleoylphosphatidylethanolamine + H2O | - |
Pseudomonas aeruginosa | ethanolamine + 1,2-dioleoyl-sn-glycero-3-phosphatidic acid | - |
? | |
| additional information | PldA is capable of using methanol, ethanol, and butanol to produce transphosphatidylation products | Pseudomonas aeruginosa | ? | - |
? | |
| oleoyllysophosphatidylethanolamine + H2O | - |
Pseudomonas aeruginosa | ethanolamine + 1,2-dioleoyl-sn-glycero-3-phosphatidic acid | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PldA | - |
Pseudomonas aeruginosa |
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Release 2023.1 (January 2023)
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