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Literature summary for 3.1.4.4 extracted from
- Identification of a sphingolipid-specific phospholipase D activity associated with the generation of phytoceramide-1-phosphate in cabbage leaves (2013), FEBS J., 280, 3797-3809.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | complete inhibition | Brassica oleracea |  |
| additional information | no inhibiton by EDTA | Brassica oleracea |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Brassica oleracea | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | absolutely dependent on, no activity without Ca2+ | Brassica oleracea | |
| Ca2+ | the enzyme is activated by Ca2+, in absence of Ca2+ it exhibits 70% of maximal activity | Brassica oleracea | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylcholine + H2O | Brassica oleracea | - |
choline + phosphatidate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
| Brassica oleracea | - |
- |
- |
| Capsella bursa-pastoris | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme partially by preparation of the 13000 rpm fraction | Arabidopsis thaliana |
| native enzyme partially by preparation of the 13000 rpm fraction | Capsella bursa-pastoris |
| native enzyme partially by preparation of the membrane fraction, solubilization with Triton X-100, ammonium sulfate fractionation, and diethylaminoethyl-cellulose chromatography | Brassica oleracea |
| native enzyme partially from cabbage leaves by acetone precipitation | Brassica oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Arabidopsis thaliana | - |
| leaf | - |
Brassica oleracea | - |
| leaf | - |
Capsella bursa-pastoris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0005 | - |
partially purified enzyme, pH 7.4, 30°C | Capsella bursa-pastoris |
| 0.0011 | - |
partially purified enzyme, pH 7.4, 30°C | Arabidopsis thaliana |
| 0.03 | 0.04 | partially purifed enzyme, pH 7.4, 30°C | Brassica oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycosylinositol phosphoceramide + H2O | - |
Arabidopsis thaliana | phytoceramide-1-phosphate + glycosylinositol | phytoceramide-1-phosphate with an alpha-hydroxy fatty acid | ? | |
| glycosylinositol phosphoceramide + H2O | - |
Brassica oleracea | phytoceramide-1-phosphate + glycosylinositol | phytoceramide-1-phosphate with an alpha-hydroxy fatty acid | ? | |
| glycosylinositol phosphoceramide + H2O | - |
Capsella bursa-pastoris | phytoceramide-1-phosphate + glycosylinositol | phytoceramide-1-phosphate with an alpha-hydroxy fatty acid | ? | |
| additional information | the enzyme does not hydrolyze glycosylinositol phosphoceramide | Brassica oleracea | ? | - |
? | |
| additional information | the enzyme hydrolyzes glycosylinositol phosphoceramide specifically, but not glycerophospholipids, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, or sphingomyelin, MALDI-TOF mass spectrometry product identification, overview | Arabidopsis thaliana | ? | - |
? | |
| additional information | the enzyme hydrolyzes glycosylinositol phosphoceramide specifically, but not glycerophospholipids, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, or sphingomyelin, MALDI-TOF mass spectrometry product identification, overview | Brassica oleracea | ? | - |
? | |
| additional information | the enzyme hydrolyzes glycosylinositol phosphoceramide specifically, but not glycerophospholipids, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, or sphingomyelin, MALDI-TOF mass spectrometry product identification, overview | Capsella bursa-pastoris | ? | - |
? | |
| phosphatidylcholine + H2O | - |
Brassica oleracea | choline + phosphatidate | - |
? | |
| phosphatidylcholine + H2O | from egg yolk | Brassica oleracea | choline + phosphatidate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GIPC-PLD | - |
Arabidopsis thaliana |
| GIPC-PLD | - |
Brassica oleracea |
| GIPC-PLD | - |
Capsella bursa-pastoris |
| PC-PLD | - |
Brassica oleracea |
| PLD | - |
Arabidopsis thaliana |
| PLD | - |
Brassica oleracea |
| PLD | - |
Capsella bursa-pastoris |
| sphingolipid-specific phospholipase D | - |
Arabidopsis thaliana |
| sphingolipid-specific phospholipase D | - |
Brassica oleracea |
| sphingolipid-specific phospholipase D | - |
Capsella bursa-pastoris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Arabidopsis thaliana |
| 30 | - |
assay at | Brassica oleracea |
| 30 | - |
assay at | Capsella bursa-pastoris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Brassica oleracea |
| 7.4 | - |
assay at | Arabidopsis thaliana |
| 7.4 | - |
assay at | Capsella bursa-pastoris |
| 7.5 | - |
- |
Brassica oleracea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 8.5 | activity range | Brassica oleracea |
| 4.5 | 7 | activity range | Brassica oleracea |
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