Literature summary for 3.1.4.4 extracted from

Knoepp, S.M.; Chahal, M.S.; Xie, Y.; Zhang, Z.; Brauner, D.J.; Hallman, M.A.; Robinson, S.A.; Han, S.; Imai, M.; Tomlinson, S.; Meier, K.E.
Effects of active and inactive phospholipase D2 on signal transduction, adhesion, migration, invasion, and metastasis in EL4 lymphoma cells (2008), Mol. Pharmacol., 74, 574-584.

Activating Compound

Activating Compound	Comment	Organism
ionomycin	-	Homo sapiens
okadaic acid	-	Homo sapiens
phorbol ester	-	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
PLD2 from PC-3 cells, stable expression of active and inactive HA-tagged PLD2 in EL4 lymphoma cells, i.e. C5 cells, devoid of endogeneous PLD1 and PLD2. Proliferation and Erk activation are unchanged in cells transfected with active PLD2, while proliferation rate is decreased in cells expressing inactive PLD2. Basal tyrosine phosphorylation of focal adhesion kinase is increased in cells expressing active PLD2, as is phosphorylation of Akt; while inactive PLD2 has no effect. Expression of active PLD2 is associated with increased spreading and elongation of cells on tissue culture plastic, whereas inactive PLD2 inhibits cell spreading. Inactive PLD2 also inhibits cell adhesion, migration, and serum-induced invasion. Cells expressing active PLD2 form metastases in syngeneic mice, as do the parental cells, cells expressing inactive PLD2 form fewer metastases than parental cells	Homo sapiens

Cloned (Comment)

Organism

PLD2 from PC-3 cells, stable expression of active and inactive HA-tagged PLD2 in EL4 lymphoma cells, i.e. C5 cells, devoid of endogeneous PLD1 and PLD2. Proliferation and Erk activation are unchanged in cells transfected with active PLD2, while proliferation rate is decreased in cells expressing inactive PLD2. Basal tyrosine phosphorylation of focal adhesion kinase is increased in cells expressing active PLD2, as is phosphorylation of Akt; while inactive PLD2 has no effect. Expression of active PLD2 is associated with increased spreading and elongation of cells on tissue culture plastic, whereas inactive PLD2 inhibits cell spreading. Inactive PLD2 also inhibits cell adhesion, migration, and serum-induced invasion. Cells expressing active PLD2 form metastases in syngeneic mice, as do the parental cells, cells expressing inactive PLD2 form fewer metastases than parental cells

Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Homo sapiens	16020	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	effects of active and inactive phospholipase D2 on signal transduction, adhesion, migration, invasion, and metastasis in EL4 lymphoma cells, overview	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	isozyme PLD2	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
DU-145 cell	-	Homo sapiens	-
additional information	isozyme PLD2 is widely expressed in mammalian cells	Homo sapiens	-
OVCAR-3 cell	-	Homo sapiens	-
PC-12K cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	effects of active and inactive phospholipase D2 on signal transduction, adhesion, migration, invasion, and metastasis in EL4 lymphoma cells, overview	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
phospholipase D2	-	Homo sapiens
PLD2	-	Homo sapiens

General Information

General Information	Comment	Organism
malfunction	PLD2 is active in lymphoma cell metastasis, cells expressing active PLD2 form metastases in syngeneic mice	Homo sapiens
physiological function	PLD2 is involved in activation of phosphorylation of focal adhesion kinase, and it plays a role in spreading and elongation of cells. Active PLD2 enhances FAK phosphorylation, Akt activation, and cell invasion in EL4 lymphoma cells, overview	Homo sapiens