Literature summary for 3.1.4.4 extracted from

Henkels, K.M.; Peng, H.J.; Frondorf, K.; Gomez-Cambronero, J.
A comprehensive model that explains the regulation of phospholipase D2 activity by phosphorylation-dephosphorylation (2010), Mol. Cell. Biol., 30, 2251-2263.

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of PLD2 in MCF-7 cells, in MTLn3 cells, or in COS-7 cells. In COS-7, cells that show the highest level of PLD2 activity, the Y415 is a prominent phosphorylation site, and JAK3 compensates the negative modulation by EGFR on phosphorylation site Y296. In MCF-7, cells that show the lowest level of PLD2 activity, the converse is the case, with phosphorylation site Y296 unable to compensate the positive modulation by phosphorylation site Y415. PLD2 activity is low in the breast cancer cell line MCF-7 because it is kept downregulated by tyrosyl phosphorylation of Y296 by EGFR kinase, overview	Mus musculus

Cloned (Comment)

Organism

overexpression of PLD2 in MCF-7 cells, in MTLn3 cells, or in COS-7 cells. In COS-7, cells that show the highest level of PLD2 activity, the Y415 is a prominent phosphorylation site, and JAK3 compensates the negative modulation by EGFR on phosphorylation site Y296. In MCF-7, cells that show the lowest level of PLD2 activity, the converse is the case, with phosphorylation site Y296 unable to compensate the positive modulation by phosphorylation site Y415. PLD2 activity is low in the breast cancer cell line MCF-7 because it is kept downregulated by tyrosyl phosphorylation of Y296 by EGFR kinase, overview

Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Mus musculus	PLD2 is regulated by phosphorylation-dephosphorylation, detailed overview	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	the enzymatic activity of phospholipase D2, PLD2, is regulated by phosphorylation-dephosphorylation with existence of activator and inhibitory sites, overview. Epidermal growth factor receptor, i.e. EGFR, JAK3, and Src with JAK3 phosphorylate an inhibitory Y296, an activator Y415, and an ambivalent Y511 site, respectively	Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	PLD2 is regulated by phosphorylation-dephosphorylation, detailed overview	Mus musculus	?	-	?

Synonyms

Synonyms	Comment	Organism
phospholipase D2	-	Mus musculus
PLD2	-	Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Mus musculus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.8	-	assay at	Mus musculus