Activating Compound | Comment | Organism | Structure |
---|---|---|---|
mastoparan | a PLD activity stimulator | Silybum marianum | |
methyl {(1S,2S)-3-oxo-2-[(2Z)-pent-2-en-1-yl]cyclopentyl}acetate | increases PLD activity | Silybum marianum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | treatment with iso-, sec- or tert-butanol has no effect on silymarin production | Silybum marianum | |
n-butanol | inhibits phosphatidic acid production by PLD, prevents silymarin elicitation by methyljasmonate or mastoparan, and also impedes its release in non-elicited cultures. Exogenous addition of phosphatidic acid reverses the inhibitory action of nBuOH, both in control and methyljasmonate-treated cultures | Silybum marianum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Silybum marianum | silymarin secretion and its elicitation by methyl jasmonate in cell cultures of Silybum marianum is mediated by phospholipase D-phosphatidic acid, overview | ? | - |
? | |
phosphatidylcholine + H2O | Silybum marianum | phosphatidic acid activates the production of and promotes accumulation of silymarin, overview | choline + phosphatidic acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Silybum marianum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-hexadecanoyl-2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)aminohexanoyl]-sn-glycero-3-phosphocholine + H2O | fluorescent substrate | Silybum marianum | ? | - |
? | |
additional information | silymarin secretion and its elicitation by methyl jasmonate in cell cultures of Silybum marianum is mediated by phospholipase D-phosphatidic acid, overview | Silybum marianum | ? | - |
? | |
phosphatidylcholine + H2O | - |
Silybum marianum | choline + phosphatidic acid | - |
? | |
phosphatidylcholine + H2O | phosphatidic acid activates the production of and promotes accumulation of silymarin, overview | Silybum marianum | choline + phosphatidic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PLD | - |
Silybum marianum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Silybum marianum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Silybum marianum |
General Information | Comment | Organism |
---|---|---|
physiological function | phospholipase D cleaves phospholipids into phosphatidic acid and free-head groups such as choline | Silybum marianum |