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Literature summary for 3.1.4.4 extracted from
- CtBP1/BARS is an activator of phospholipase D1 necessary for agonist-induced macropinocytosis (2009), EMBO J., 28, 1197-1207.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Arf1 protein | activates PLD, acts synergistically with CtBP1/BARS in activation of PLD, dependent on GTPgammaS or GTP but not on GDP, overview | Rattus norvegicus | |
| CtBP1/BARS | a short splice variant of the CtBP1 gene and a physiological activator of PLD1 required in agonist-induced macropinocytosis. Stimulation of cells by serum or EGF results in the association of CtBP1/BARS with PLD1, which is specifically blocked by 1-butanol. CtBP1/BARS acts synergistically with ARF1 protein in activation of PLD, dependent on GTPgammaS or GTP but not on GDP, overview | Rattus norvegicus | |
| additional information | activation mechanism of PLD in macropinocytosis, overview | Rattus norvegicus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| co-expression of the respective wild-type PLD isoforms or lipase-negative (LN)-PLD1 or LN-PLD2inactive mutants in PLD1 or PLD2 KO mutant cells, respectively | Rattus norvegicus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | the inhibition of EGF-induced macropinocytosis caused either by PLD1 or PLD2 knockdown is rescued by the co-expression of the respective wild-type PLD isoforms, whereas the expression of a lipase inactive mutant, lipase-negative (LN)-PLD1 or LN-PLD2, does not restore macropinocytotic activity | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-butanol | - |
Rattus norvegicus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Rattus norvegicus | PLD performs two different reactions: a hydrolytic reaction and a transphosphatidylation reaction, 1-butanol serves as acceptor in the transphosphatidylation reaction, while 2-butanol does not. PLD-catalysed PtdOH formation may be necessary for EGF-induced macropinocytosis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
isozymes PLD1 and PLD2 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | PLD performs two different reactions: a hydrolytic reaction and a transphosphatidylation reaction, 1-butanol serves as acceptor in the transphosphatidylation reaction, while 2-butanol does not. PLD-catalysed PtdOH formation may be necessary for EGF-induced macropinocytosis | Rattus norvegicus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| phospholipase D1 | - |
Rattus norvegicus |
| PLD1 | - |
Rattus norvegicus |
| PLD2 | - |
Rattus norvegicus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | vesicular trafficking such as macropinocytosis is a dynamic process that requires coordinated interactions between specialized proteins and lipids that involves PLD and its physiological activator, the fission protein CtBP1/BARS in agonist-induced macropinocytosis, overview. Molecular mechanisms of lipid remodelling regulation during macropinocytosis. PLD-catalysed PtdOH formation may be necessary for EGF-induced macropinocytosis | Rattus norvegicus |
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