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Literature summary for 3.1.4.4 extracted from

  • Hamdi, S.M.; Cariven, C.; Coronas, S.; Malet, N.; Chap, H.; Perret, B.; Salles, J.P.; Record, M.
    Potential role of phospholipase D2 in increasing interleukin-2 production by T-lymphocytes through activation of mitogen-activated protein kinases ERK1/ERK2 (2008), Biochim. Biophys. Acta, 1781, 263-269.
    View publication on PubMed
Search for more literature for 3.1.4.4

Activating Compound

Activating Compound Comment Organism Structure
additional information PLD2 activity is enhanced by PMA/ionomycin stimulation Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
stable expression of PLD2 in Jurkat cells. Overexpression of a dominant negative PLD2 attenuates the early and sustained increase in ERK1/2 phosphorylation induced by PLD2, overview. Phosphorylation of ERK1/2 promoted by PMA-ionomycin in transfected Jurkat cells is inhibited by 1-butanol Homo sapiens
stable expression of PLD2 in T hybridoma 2B4 cell line. Overexpression of a dominant negative PLD2 attenuates the early and sustained increase in ERK1/2 phosphorylation induced by PLD2, overview Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information interleukin-2 production evoked by PMA/ionomycin stimulation or CD3/CD28 engagement is enhanced in the two T-cell lines overexpressing PLD2 Mus musculus
additional information interleukin-2 production evoked by PMA/ionomycin stimulation or CD3/CD28 engagement is enhanced in the two T-cell lines overexpressing PLD2. hPLD2 overexpression results in an increase of ERK1/2 phosphorylation by about 50% within 60 min Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1-butanol 1-butanol inhibits PLD2 and is potent in cancelling ERK1/2 activation Homo sapiens
1-butanol 1-butanol inhibits PLD2 and is potent in cancelling ERK1/2 activation Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Mus musculus
Ca2+
-
 Homo sapiens
Mg2+
-
 Mus musculus
Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphatidylcholine + H2O Mus musculus hydrolysis of phosphatidylcholine by phospholipase D leads to the generation of phosphatidic acid, PA, which is itself a source of diacylglycerol. PLD2 emerges as an early player upstream of the Ras-MAPK-IL-2 pathway in T-cells via PA and DAG production choline + phosphatidic acid
-
 ?
phosphatidylcholine + H2O Homo sapiens hydrolysis of phosphatidylcholine by phospholipase D leads to the generation of phosphatidic acid, which is itself a source of diacylglycerol. PLD2 emerges as an early player upstream of the Ras-MAPK-IL-2 pathway in T-cells via phosphatidic acid and diacylglycerol production choline + phosphatidic acid
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 isozyme PLD2
-
Mus musculus
-
 isozyme PLD2
-

Source Tissue

Source Tissue Comment Organism Textmining
JURKAT cell PLD2 is the main isoform present in the Jurkat cell line Homo sapiens
-
K-562 cell a myeloid cell line Homo sapiens
-
KARPAS-422 cell a B lymphoid cell line Homo sapiens
-
KG-1A cell a myeloid cell line Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information PLD assay on whole haematopoietic cells Mus musculus ?
-
 ?
additional information PLD assay on whole hematopoietic cells Homo sapiens ?
-
 ?
phosphatidylcholine + H2O
-
 Mus musculus choline + phosphatidate
-
 ?
phosphatidylcholine + H2O
-
 Homo sapiens choline + phosphatidate
-
 ?
phosphatidylcholine + H2O hydrolysis of phosphatidylcholine by phospholipase D leads to the generation of phosphatidic acid, PA, which is itself a source of diacylglycerol. PLD2 emerges as an early player upstream of the Ras-MAPK-IL-2 pathway in T-cells via PA and DAG production Mus musculus choline + phosphatidic acid
-
 ?
phosphatidylcholine + H2O hydrolysis of phosphatidylcholine by phospholipase D leads to the generation of phosphatidic acid, which is itself a source of diacylglycerol. PLD2 emerges as an early player upstream of the Ras-MAPK-IL-2 pathway in T-cells via phosphatidic acid and diacylglycerol production Homo sapiens choline + phosphatidic acid
-
 ?

Synonyms

Synonyms Comment Organism
phospholipase D2
-
 Mus musculus
phospholipase D2
-
 Homo sapiens
PLD
-
 Mus musculus
PLD
-
 Homo sapiens
PLD2
-
 Mus musculus
PLD2
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Mus musculus
7.4
-
 assay at Homo sapiens

Expression

Organism Comment Expression
Homo sapiens an increase in PLD2 expression correlates with an enhanced ERK-1/-2 activation following PMA/ionomycin stimulation up

General Information

General Information Comment Organism
metabolism phosphatidic acid is a source of diacylglycerol, the two versatile lipid second messengers are at the centre of a phospholipid signalling network and as such are involved in several cellular functions Mus musculus
metabolism phosphatidic acid is a source of diacylglycerol, the two versatile lipid second messengers are at the centre of a phospholipid signalling network and as such are involved in several cellular functions Homo sapiens
physiological function PLD acting upstream of the MAP kinases ERK1/2 may play a key role in the regulation of IL-2 production by stimulated Jurkat cells. PLD2 promotes an early and sustained increase in ERK1/2 phosphorylation in recombinant T-cell lines, which is inhibited by 1-butanol Homo sapiens
physiological function PLD2 promotes an early and sustained increase in ERK1/2 phosphorylation in recombinant T-cell lines, which is inhibited by 1-butanol. Mus musculus