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Literature summary for 3.1.4.4 extracted from

  • Mander, P.; Simkhada, J.R.; Cho, S.S.; Park, S.J.; Choi, H.S.; Lee, H.C.; Sohng, J.K.; Yoo, J.C.
    A novel Ca2+-dependent phospholipase D from Streptomyces tendae, possessing only hydrolytic activity (2009), Arch. Pharm. Res., 32, 1461-1467.
    View publication on PubMed
Search for more literature for 3.1.4.4

Activating Compound

Activating Compound Comment Organism Structure
CHAPS activates 1.8fold at 1.5% Streptomyces tendae
deoxycholic acid activates 2.2fold at 1.5% Streptomyces tendae
polyethylene-4-laurylether activates 2.1fold at 1.5% Streptomyces tendae
Triton X-100 activates 2.65fold at 1.5% Streptomyces tendae
Tween 80 activates 2fold at 1.5% Streptomyces tendae
Tween-20 activates 2.3fold at 1.5% Streptomyces tendae

Inhibitors

Inhibitors Comment Organism Structure
EDTA complete inhibition at 2 mM Streptomyces tendae
N-laurylsarcosine 75% inhibition at 1.5% Streptomyces tendae
SDS complete inhibition at 1.5% Streptomyces tendae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.95
-
 phosphatidylcholine pH 8.0, 60°C Streptomyces tendae

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Streptomyces tendae
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ absolutely required, best at 2 mM, cannot be substituted by other metal ions, overview Streptomyces tendae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 x * 43000, SDS-PAGE Streptomyces tendae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphatidylcholine + H2O Streptomyces tendae
-
 choline + phosphatidic acid
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces tendae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 4fold from culture supernatant by ammonium sulfate fractionation, dialysis, ultrafiltration, gel filtration and anion exchange chromatography Streptomyces tendae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 the purified native enzyme shows a specific activity of 18 U/ml Streptomyces tendae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information PLDSt cannot catalyze transphosphatidylation of glycerol, L-serine, myo-inositol and ethanolamine, the Streptomyces tendae PLD possesses only hydrolytic activity Streptomyces tendae ?
-
 ?
phosphatidylcholine + H2O
-
 Streptomyces tendae choline + phosphatidic acid
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Streptomyces tendae

Synonyms

Synonyms Comment Organism
Ca2+-dependent phospholipase D
-
 Streptomyces tendae
phosphatidylcholine choline hydrolase
-
 Streptomyces tendae
PLD
-
 Streptomyces tendae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 hydrolase activity Streptomyces tendae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 70 purified enzyme, 1.5 h, over 90% of maximal activity Streptomyces tendae
65
-
 90 min, purified enzyme, stable Streptomyces tendae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 hydrolase activity Streptomyces tendae

pH Range

pH Minimum pH Maximum Comment Organism
6 11 activity range, profile, overview Streptomyces tendae

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5 11 purified enzyme, 24 h, over 80% of maximal activity within that range, over 95% at pH 8.0-10.0 Streptomyces tendae