Application | Comment | Organism |
---|---|---|
molecular biology | knockout and overexpression of PLDalpha3 alter plant response to salinity and water deficit. Alterations of PLDalpha3 result in changes in phosphatidic acid level and membrane lipid composition. PLDalpha3-knockout plants display increased sensitivities to salinity and water deficiency and also tend to induce abscisic acid-responsive genes more readily than wild-type plants, whereas PLDalpha3-overexpressed plants have decreased sensitivities. PLDalpha3-knockout plants flower later than wild-type plants in slightly dry conditions, whereas PLDalpha3-overexpressed plants flower earlier | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
expressed in Arabidopsis thaliana as a His-tagged fusion protein | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | knockout and overexpression of PLDalpha3 alter plant response to salinity and water deficit. Alterations of PLDalpha3 result in changes in phosphatidic acid level and membrane lipid composition. PLDalpha3-knockout plants display increased sensitivities to salinity and water deficiency and also tend to induce abscisic acid-responsive genes more readily than wild-type plants, whereas PLDalpha3-overexpressed plants have decreased sensitivities. PLDalpha3-knockout plants flower later than wild-type plants in slightly dry conditions, whereas PLDalpha3-overexpressed plants flower earlier | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q38882 | - |
- |
Arabidopsis thaliana | Q9C888 | - |
- |
Purification (Comment) | Organism |
---|---|
purified from plant proteins by immunoaffinity column chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bud | expression is 1000fold higher than that of isoenzyme PLDalpha3 | Arabidopsis thaliana | - |
bud | expression is 1000fold lower than that of isoenzyme PLDalpha1 | Arabidopsis thaliana | - |
flower | expression is 1000fold higher than that of isoenzyme PLDalpha3 | Arabidopsis thaliana | - |
flower | expression is 1000fold lower than that of isoenzyme PLDalpha1 | Arabidopsis thaliana | - |
leaf | expression is 1000fold higher than that of isoenzyme PLDalpha3 | Arabidopsis thaliana | - |
leaf | expression is 1000fold lower than that of isoenzyme PLDalpha1 | Arabidopsis thaliana | - |
root | expression is 1000fold higher than that of isoenzyme PLDalpha3 | Arabidopsis thaliana | - |
root | expression is 1000fold lower than that of isoenzyme PLDalpha1 | Arabidopsis thaliana | - |
silique | expression is 1000fold higher than that of isoenzyme PLDalpha3 | Arabidopsis thaliana | - |
silique | expression is 1000fold lower than that of isoenzyme PLDalpha1 | Arabidopsis thaliana | - |
stem | expression is 1000fold higher than that of isoenzyme PLDalpha3 | Arabidopsis thaliana | - |
stem | expression is 1000fold lower than that of isoenzyme PLDalpha1 | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylcholine + H2O | enzyme shows the highest activity toward phosphatidylcholine and the lowest toward phosphatidylserine | Arabidopsis thaliana | phosphatidate + choline | - |
? | |
phosphatidylglycerol + H2O | enzyme shows the highest activity toward phosphatidylcholine and the lowest toward phosphatidylserine | Arabidopsis thaliana | phosphatidate + glycerol | - |
? | |
phosphatidylserine + H2O | enzyme shows the highest activity toward phosphatidylcholine and the lowest toward phosphatidylserine | Arabidopsis thaliana | phosphatidate + serine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
phospholipase Dalpha1 | - |
Arabidopsis thaliana |
phospholipase Dalpha3 | - |
Arabidopsis thaliana |
PLDalpha1 | - |
Arabidopsis thaliana |
PLDalpha3 | - |
Arabidopsis thaliana |