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Literature summary for 3.1.4.4 extracted from

  • Hong, Y.; Pan, X.; Welti, R.; Wang, X.
    Phospholipase Dalpha3 is involved in the hyperosmotic response in Arabidopsis (2008), Plant Cell, 20, 803-816.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
molecular biology knockout and overexpression of PLDalpha3 alter plant response to salinity and water deficit. Alterations of PLDalpha3 result in changes in phosphatidic acid level and membrane lipid composition. PLDalpha3-knockout plants display increased sensitivities to salinity and water deficiency and also tend to induce abscisic acid-responsive genes more readily than wild-type plants, whereas PLDalpha3-overexpressed plants have decreased sensitivities. PLDalpha3-knockout plants flower later than wild-type plants in slightly dry conditions, whereas PLDalpha3-overexpressed plants flower earlier Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Arabidopsis thaliana as a His-tagged fusion protein Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information knockout and overexpression of PLDalpha3 alter plant response to salinity and water deficit. Alterations of PLDalpha3 result in changes in phosphatidic acid level and membrane lipid composition. PLDalpha3-knockout plants display increased sensitivities to salinity and water deficiency and also tend to induce abscisic acid-responsive genes more readily than wild-type plants, whereas PLDalpha3-overexpressed plants have decreased sensitivities. PLDalpha3-knockout plants flower later than wild-type plants in slightly dry conditions, whereas PLDalpha3-overexpressed plants flower earlier Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q38882
-
-
Arabidopsis thaliana Q9C888
-
-

Purification (Commentary)

Purification (Comment) Organism
purified from plant proteins by immunoaffinity column chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
bud expression is 1000fold higher than that of isoenzyme PLDalpha3 Arabidopsis thaliana
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bud expression is 1000fold lower than that of isoenzyme PLDalpha1 Arabidopsis thaliana
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flower expression is 1000fold higher than that of isoenzyme PLDalpha3 Arabidopsis thaliana
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flower expression is 1000fold lower than that of isoenzyme PLDalpha1 Arabidopsis thaliana
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leaf expression is 1000fold higher than that of isoenzyme PLDalpha3 Arabidopsis thaliana
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leaf expression is 1000fold lower than that of isoenzyme PLDalpha1 Arabidopsis thaliana
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root expression is 1000fold higher than that of isoenzyme PLDalpha3 Arabidopsis thaliana
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root expression is 1000fold lower than that of isoenzyme PLDalpha1 Arabidopsis thaliana
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silique expression is 1000fold higher than that of isoenzyme PLDalpha3 Arabidopsis thaliana
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silique expression is 1000fold lower than that of isoenzyme PLDalpha1 Arabidopsis thaliana
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stem expression is 1000fold higher than that of isoenzyme PLDalpha3 Arabidopsis thaliana
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stem expression is 1000fold lower than that of isoenzyme PLDalpha1 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidylcholine + H2O enzyme shows the highest activity toward phosphatidylcholine and the lowest toward phosphatidylserine Arabidopsis thaliana phosphatidate + choline
-
?
phosphatidylglycerol + H2O enzyme shows the highest activity toward phosphatidylcholine and the lowest toward phosphatidylserine Arabidopsis thaliana phosphatidate + glycerol
-
?
phosphatidylserine + H2O enzyme shows the highest activity toward phosphatidylcholine and the lowest toward phosphatidylserine Arabidopsis thaliana phosphatidate + serine
-
?

Synonyms

Synonyms Comment Organism
phospholipase Dalpha1
-
Arabidopsis thaliana
phospholipase Dalpha3
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Arabidopsis thaliana
PLDalpha1
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Arabidopsis thaliana
PLDalpha3
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Arabidopsis thaliana