Literature summary for 3.1.4.4 extracted from

Fedeli, C.; Carrea, G.; Monti, D.
On the effects of site-specific mutations on activity and expression of the Streptomyces PMF phospholipase D (2006), J. Mol. Catal. B, 41, 1-7.

No PubMed abstract available

Search for more literature for 3.1.4.4

Application

Application	Comment	Organism
synthesis	production of enzyme in Escherichia coli is strongly influenced by the catalytic activity as cytotoxicity of protein for host is strictly coorelated to enzyme activity. Synthesis of inactive mutants gives recovery yields 5-10 times higher than for wild-type	Streptomyces sp.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli. Cytotoxicity of protein for host is strictly coorelated to enzyme activity	Streptomyces sp.

Protein Variants

Protein Variants	Comment	Organism
H167N	inactive	Streptomyces sp.
H440N	inactive	Streptomyces sp.
K169S	inactive	Streptomyces sp.
K442S	inactive	Streptomyces sp.

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	recombinant protein	Streptomyces sp.	-	-

Organism

Organism	UniProt	Comment	Textmining
Streptomyces sp.	-	-	-

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Release 2023.1 (January 2023)