Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme is independent of phosphatidylinositol-4,5-bisphosphate | Brassica juncea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1.5 mM, 30% loss of activity | Brassica juncea | |
additional information | enzyme completely loses activity upon dialysis | Brassica juncea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | about 40% stimulation in presence of at least 2 mM | Brassica juncea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica juncea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial. Enzyme completely loses activity upon dialysis | Brassica juncea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Brassica juncea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
168.5 | - |
pH 5.5, 37°C, crude extract of seed | Brassica juncea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylcholine + H2O | - |
Brassica juncea | 1,2-diacylglycerophosphate + choline | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Brassica juncea |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 70 | more than 70% of maximum activity | Brassica juncea |
30 | 60 | more than 94% of maximum activity | Brassica juncea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
30 min, almost 100% rersidual activity | Brassica juncea |
70 | - |
30 min, 55% residual activity | Brassica juncea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Brassica juncea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | more than 73% of maximum activity | Brassica juncea |