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Literature summary for 3.1.4.4 extracted from

  • Stumpe, S.; Koenig, S.; Ulbrich-Hofmann, R.
    Insights into the structure of plant alpha-type phospholipase D (2007), FEBS J., 274, 2630-2640.
    View publication on PubMed
Search for more literature for 3.1.4.4

Crystallization (Commentary)

Crystallization (Comment) Organism
protein forms a barrel-shaped monomer with loosely structured tops. Presence of a-helical and dominant beta-structural elements with tight packing of aromatic residues in the core of the protein Brassica oleracea

Protein Variants

Protein Variants Comment Organism
A426F less than 1% of wild-type activity with substrate phosphatidyl-p-nitrophenol Brassica oleracea
H443A less than 1% of wild-type activity with substrate phosphatidyl-p-nitrophenol Brassica oleracea
L483H less than 1% of wild-type activity with substrate phosphatidyl-p-nitrophenol Brassica oleracea

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ substantial reduction of stability due to salt-induced aggregation Brassica oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 1 * 57000, SDS-PAGE, crystallization data Brassica oleracea

Organism

Organism UniProt Comment Textmining
Brassica oleracea P55939 isoform PLDalpha2
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
59
-
 wild-type, pH 5.5, 30°C Brassica oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidyl-p-nitrophenol + H2O
-
 Brassica oleracea phosphatidic acid + p-nitrophenol
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 57000, SDS-PAGE, crystallization data Brassica oleracea