Cloned (Comment) | Organism |
---|---|
- |
Shewanella sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 2.5 mM, complete loss of both hydrolytic activity and transphosphatidylation | Shewanella sp. | |
Co2+ | 2.5 mM, complete loss of both hydrolytic activity and transphosphatidylation | Shewanella sp. | |
Cu2+ | 2.5 mM, complete loss of both hydrolytic activity and transphosphatidylation | Shewanella sp. | |
Fe3+ | 2.5 mM, complete loss of both hydrolytic activity and transphosphatidylation | Shewanella sp. | |
Mg2+ | 2.5 mM, inhibitory to both hydrolytic activity and transphosphatidylation | Shewanella sp. | |
Mn2+ | 2.5 mM, complete loss of both hydrolytic activity and transphosphatidylation | Shewanella sp. | |
Ni2+ | 2.5 mM, complete loss of both hydrolytic activity and transphosphatidylation | Shewanella sp. | |
Zn2+ | 2.5 mM, complete loss of both hydrolytic activity and transphosphatidylation | Shewanella sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | presence of Ca2+ enhances 1.6fold hydrolytic activiy and 6fold transphosphatidylation | Shewanella sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64000 | - |
x * 64000, SDS-PAGE | Shewanella sp. |
Organic Solvent | Comment | Organism |
---|---|---|
Ethanol | enzyme shows high activity to the non-micelle form of phosphatidylcholine in an aequeous solution containing methanol, ethanol, iso-propanol, or n-propanol | Shewanella sp. |
isopropanol | enzyme shows high activity to the non-micelle form of phosphatidylcholine in an aequeous solution containing methanol, ethanol, isopropanol, or n-propanol | Shewanella sp. |
Methanol | assay in presence of 50% ethanol | Shewanella sp. |
n-propanol | enzyme shows high activity to the non-micelle form of phosphatidylcholine in an aequeous solution containing methanol, ethanol, isopropanol, or n-propanol | Shewanella sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Shewanella sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Shewanella sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.076 | - |
pH 7.8, 25°C, presence of 50% methanol | Shewanella sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme exhibits a ratio of hydrolytic activity to transphosphatidylation of 2.5 | Shewanella sp. | ? | - |
? | |
phosphatidylcholine + H2O | high activity to the non-micelle form of phosphatidylcholine in an aequeous solution containing methanol, ethanol, isopropanol, or n-propanol. In absence of alcohol, hydrolytic activity is weak, and no transphosphatidylation activity is detected | Shewanella sp. | 1,2-diacylglycerophosphate + choline | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 64000, SDS-PAGE | Shewanella sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
presence of Ca2+ | Shewanella sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | - |
6.5% of maximum activity | Shewanella sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
stable below | Shewanella sp. |
40 | - |
rather labile above | Shewanella sp. |
60 | - |
rapid loss of activity | Shewanella sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Shewanella sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | enzyme is labile in the low pH range | Shewanella sp. |