Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.4.4 extracted from

  • Henage, L.G.; Exton, J.H.; Brown, H.A.
    Kinetic analysis of a mammalian phospholipase D: Allosteric modulation by monomeric GTPases, protein kinase C, and polyphosphoinositides (2006), J. Biol. Chem., 281, 3408-3417.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.4.4

Activating Compound

Activating Compound Comment Organism Structure
Arf-1 the catalytic activator stimulates PLD1 by enhancing the catalytic constant kcat. Full-length enzyme and N-terminally truncated enzyme are strongly activated by Arf-1-GTPgammaS Rattus norvegicus
Cdc42 stimulation of full-length enzyme and N-terminally truncated enzyme by GTPgammaS-loaded Cdc42 Rattus norvegicus
phosphatidylinositol 4,5-bisphosphate stimulation of full-length enzyme and N-terminally truncated enzyme Rattus norvegicus
protein kinase Calpha activation of PLD1 involves N- and C-terminal PLD domains Rattus norvegicus
Rac1 stimulation of full-length enzyme and N-terminally truncated enzyme by GTPgammaS-loaded Rac1 Rattus norvegicus
RhoA stimulation of full-length enzyme and N-terminally truncated enzyme by GTPgammaS-loaded RhoA Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
full-length enzyme and N-terminally truncated enzyme PLD1 expressed as a maltose binding domain fusion protein Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information KM-value for phosphatidylcholine (unstimulated) is 33mol%, full-length enzyme Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus PLD1 is a signaling node, in which integration of convergent signals occurs within discrete locales of the cellular membrane ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
full-length enzyme and N-terminally truncated enzyme PLD1 expressed as a maltose binding domain fusion protein Rattus norvegicus

Storage Stability

Storage Stability Organism
-80°C, purified enzyme retains activity for more than 1 year Rattus norvegicus
4°C, purified enzyme retains high activity for 2-3 days Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information PLD1 is a signaling node, in which integration of convergent signals occurs within discrete locales of the cellular membrane Rattus norvegicus ?
-
 ?
phosphatidylcholine + H2O
-
 Rattus norvegicus 1,2-diacylglycerophosphate + choline
-
 ?

Synonyms

Synonyms Comment Organism
PLD1
-
 Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.067
-
 phosphatidylcholine unstimulated full lenth enzyme Rattus norvegicus
0.08
-
 phosphatidylcholine N-terminally truncated enzyme PLD1, stimulated by PKCalpha Rattus norvegicus
0.1
-
 phosphatidylcholine N-terminally truncated enzyme PLD1, stimulated by Cdc42 Rattus norvegicus
0.1
-
 phosphatidylcholine N-terminally truncated enzyme PLD1, stimulated by PKCalpha + Rac1 Rattus norvegicus
0.1
-
 phosphatidylcholine N-terminally truncated enzyme PLD1, stimulated by PKCalpha + RhoA Rattus norvegicus
0.1
-
 phosphatidylcholine N-terminally truncated enzyme PLD1, unstimulated Rattus norvegicus
0.117
-
 phosphatidylcholine N-terminally truncated enzyme PLD1, stimulated by PKCalpha + Cdc42 Rattus norvegicus
0.13
-
 phosphatidylcholine N-terminally truncated enzyme PLD1, stimulated by Rac1 Rattus norvegicus
0.17
-
 phosphatidylcholine N-terminally truncated enzyme PLD1, stimulated by RhoA Rattus norvegicus
0.18
-
 phosphatidylcholine full length enzyme stimulated by RhoA Rattus norvegicus
0.27
-
 phosphatidylcholine full length enzyme stimulated by Arf-1 Rattus norvegicus
0.37
-
 phosphatidylcholine N-terminally truncated enzyme PLD1, stimulated by Arf-1 Rattus norvegicus
0.37
-
 phosphatidylcholine N-terminally truncated enzyme PLD1, stimulated by Arf-1 + Cdc42 Rattus norvegicus
0.37
-
 phosphatidylcholine N-terminally truncated enzyme PLD1, stimulated by Arf-1 + Rac1 Rattus norvegicus
0.43
-
 phosphatidylcholine N-terminally truncated enzyme PLD1, stimulated by Arf1 + PKCalpha Rattus norvegicus
0.45
-
 phosphatidylcholine N-terminally truncated enzyme PLD1, stimulated by Arf1 + RhoA Rattus norvegicus
0.93
-
 phosphatidylcholine full length enzyme stimulated by PKCalpha Rattus norvegicus