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Literature summary for 3.1.4.4 extracted from
- Phosphohydrolase and transphosphatidylation reactions of two Streptomyces phospholipase D enzymes: Covalent versus noncovalent catalysis (2003), Protein Sci., 12, 2087-2098.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| phosphatidic acid | up to 2fold | Streptomyces chromofuscus |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Streptomyces chromofuscus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C123A | enzymatically inactive, secondary structure and thermostability similar to wild type | Streptomyces chromofuscus |
| C123S | enzymatically inactive, secondary structure and thermostability similar to wild type | Streptomyces chromofuscus |
| H171A | 17% of wild type activity | Streptomyces chromofuscus |
| H187A | dramatic decrease in activity | Streptomyces chromofuscus |
| H200A | dramatic decrease in activity | Streptomyces chromofuscus |
| H266A | 17% of wild type activity, activation by phosphatidic acid up to 12fold | Streptomyces chromofuscus |
| additional information | truncation of C-terminal 530 bp, no enzymic activtiy probably due o misfolding | Streptomyces chromofuscus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.16 | - |
1,2-dibutyl-sn-glycero-3-phosphocholine | pH 7.5, in presence of 5 mM Ca2+ | Streptomyces chromofuscus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces chromofuscus | Q8KRU5 | ATCC type strain | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Streptomyces chromofuscus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13 | - |
pH 7.5, in presence of 5 mM Ca2+ | Streptomyces chromofuscus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-dibutyl-sn-glycero-3-phosphocholine + H2O | - |
Streptomyces chromofuscus | choline + ? | - |
? | |
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