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Literature summary for 3.1.4.4 extracted from

  • Abousalham, A.; Riviere, M.; Teissere, M.; Verger, R.
    Improved purification and biochemical characterization of phospholipase D from cabbage (1993), Biochim. Biophys. Acta, 1158, 1-7.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ complete inhibition Glycine max
Cu2+ complete inhibition Glycine max
dithiobis-(2-nitrobenzoic acid)
-
Glycine max
EDTA
-
Glycine max
Mg2+
-
Glycine max
Mn2+
-
Glycine max
p-chloromercuribenzoate complete inhibition Glycine max
Sr2+
-
Glycine max
Zn2+
-
Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6
-
phosphatidylcholine
-
Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Glycine max 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 20-100 mM required for maximal activity in plants Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
92000
-
gel filtration, SDS-PAGE Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phospholipid + H2O Glycine max phosphoric ester hydrolysis phosphatidic acid + alcohol
-
?

Organism

Organism UniProt Comment Textmining
Glycine max
-
soybean
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Glycine max
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidylcholine + H2O
-
Glycine max 1,2-diacylglycerophosphate + choline
-
?
phospholipid + H2O phosphoric ester hydrolysis Glycine max phosphatidic acid + alcohol
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 7.5 Ca2+ dependent Glycine max