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Literature summary for 3.1.4.4 extracted from
- Purification of Streptomyces chromofuscus phospholipase D by hydrophobic affinity chromatography on palmitoyl cellulose (1979), J. Biochem., 85, 79-95.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| albumin | - |
Streptomyces chromofuscus | |
| ethyl ether | - |
Streptomyces chromofuscus |  |
| sodium deoxycholate | - |
Streptomyces chromofuscus | |
| Triton X-100 | - |
Streptomyces chromofuscus | |
General Stability
| General Stability | Organism |
|---|---|
| albumin increases stability of the enzyme | Streptomyces chromofuscus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | complete inhibition | Streptomyces chromofuscus | |
| Co2+ | complete inhibition | Streptomyces chromofuscus | |
| EDTA | - |
Streptomyces chromofuscus | |
| Mg2+ | stimulates in the absence of Triton X-100 | Streptomyces chromofuscus | |
| Mn2+ | - |
Streptomyces chromofuscus | |
| SDS | - |
Streptomyces chromofuscus | |
| Sn2+ | complete inhibition | Streptomyces chromofuscus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.56 | - |
sphingomyelin | - |
Streptomyces chromofuscus | |
| 1.43 | - |
phosphatidylcholine | - |
Streptomyces chromofuscus | |
| 1.67 | - |
lysophosphatidylcholine | - |
Streptomyces chromofuscus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Streptomyces chromofuscus | |
| Mg2+ | stimulates in the absence of Triton X-100 | Streptomyces chromofuscus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | 57000 | gel filtration, SDS-PAGE | Streptomyces chromofuscus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phospholipid + H2O | Streptomyces chromofuscus | phosphoric ester hydrolysis | phosphatidic acid + alcohol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces chromofuscus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity | Streptomyces chromofuscus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture supernatant | - |
Streptomyces chromofuscus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lysophosphatidylcholine + H2O | - |
Streptomyces chromofuscus | monoacylglycerophosphate + choline | - |
? | |
| phosphatidylcholine + H2O | - |
Streptomyces chromofuscus | 1,2-diacylglycerophosphate + choline | - |
? | |
| phosphatidylserine + H2O | - |
Streptomyces chromofuscus | 1,2-diacylglycerophosphate + serine | - |
? | |
| phospholipid + H2O | phosphoric ester hydrolysis | Streptomyces chromofuscus | phosphatidic acid + alcohol | - |
? | |
| sphingomyelin + H2O | - |
Streptomyces chromofuscus | N-acylsphingosylphosphate + choline | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
50% acivity | Streptomyces chromofuscus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Streptomyces chromofuscus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | - |
Streptomyces chromofuscus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
best stability | Streptomyces chromofuscus |
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